{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T14:04:25Z","timestamp":1784642665348,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,4,9]],"date-time":"2014-04-09T00:00:00Z","timestamp":1397001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","award":["APP1003478"],"award-info":[{"award-number":["APP1003478"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Spinal pseudarthrosis is a well described complication of spine fusion surgery in NF1 patients. Reduced bone formation and excessive resorption have been described in NF1 and anti\u2010resorptive agents may be advantageous in these individuals. In this study, 16 wild type and 16 <jats:italic>Nf1<\/jats:italic><jats:sup><jats:italic>+\/\u2212<\/jats:italic><\/jats:sup> mice were subjected to posterolateral fusion using collagen sponges containing 5\u2009\u00b5g rhBMP\u20102 introduced bilaterally. Mice were dosed twice weekly with 0.02\u2009mg\/kg zoledronic acid (ZA) or sterile saline. The fusion mass was assessed for bone volume (BV) and bone mineral density (BMD) by microCT. Co\u2010treatment using rhBMP\u20102 and ZA produced a significant increase (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) in BV of the fusion mass compared to rhBMP\u20102 alone in both wild type mice (+229%) and <jats:italic>Nf1<\/jats:italic><jats:sup><jats:italic>+\/\u2212<\/jats:italic><\/jats:sup> mice (+174%). Co\u2010treatment also produced a significantly higher total BMD of the fusion mass compared to rhBMP\u20102 alone in both groups (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Despite these gains with anti\u2010resorptive treatment, <jats:italic>Nf1<\/jats:italic><jats:sup><jats:italic>+\/\u2212<\/jats:italic><\/jats:sup> deficient mice still generated less bone than wild type controls. TRAP staining on histological sections indicated an increased osteoclast surface\/bone surface (Oc.S\/BS) in <jats:italic>Nf1<\/jats:italic><jats:sup><jats:italic>+\/\u2212<\/jats:italic><\/jats:sup> mice relative to wild type mice, and this was reduced with ZA treatment. \u00a9 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:1090\u20131094, 2014.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jor.22628","type":"journal-article","created":{"date-parts":[[2014,4,9]],"date-time":"2014-04-09T16:01:40Z","timestamp":1397059300000},"page":"1090-1094","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Maximizing bone formation in posterior spine fusion using rhBMP\u20102 and zoledronic acid in wild type and NF1 deficient mice"],"prefix":"10.1002","volume":"32","author":[{"given":"Justin","family":"Bobyn","sequence":"first","affiliation":[{"name":"The Centre for Children's Bone Health Sydney Children's Hospital Network Sydney Australia"},{"name":"Discipline of Paediatrics and Child Health, Faculty of Medicine University of Sydney Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Rasch","sequence":"additional","affiliation":[{"name":"The Centre for Children's Bone Health Sydney Children's Hospital Network Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikulec","family":"Kathy","sequence":"additional","affiliation":[{"name":"The Centre for Children's Bone Health Sydney Children's Hospital Network Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"Little","sequence":"additional","affiliation":[{"name":"The Centre for Children's Bone Health Sydney Children's Hospital Network Sydney Australia"},{"name":"Discipline of Paediatrics and Child Health, Faculty of Medicine University of Sydney Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Schindeler","sequence":"additional","affiliation":[{"name":"The Centre for Children's Bone Health Sydney Children's Hospital Network Sydney Australia"},{"name":"Discipline of Paediatrics and Child Health, Faculty of Medicine University of Sydney Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,4,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2134734"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(70)80472-3"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/088307380201700812"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.F.00836"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1302\/0301-620X.58B3.956246","article-title":"Pseudoarthrosis after spinal fusion for scoliosis","volume":"58","author":"McMaster M","year":"1976","journal-title":"J Bone Joint Surg Br"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1097\/00003086-198908000-00007","article-title":"Pitfalls of spinal deformities associated with neurofibromatosis in children","volume":"245","author":"Crawford AH.","year":"1989","journal-title":"Clin Orthop Relat Res"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-005-1940-2"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.bpb.0000271310.87997.ae"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2007.11.006"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20628"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.528"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.93B8.25940"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20481"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11832-010-0293-3"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1749-799X-8-2"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.9.1019"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-196850050-00004"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2006.03.004"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2011.04.023"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.33045"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2005.01.022"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.89B4.18301"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2013.06.030"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22121"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199907)46:1<51::AID-JBM6>3.0.CO;2-I"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00337-5"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(97)00248-2"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007632-200212010-00005"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjor.22628","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjor.22628","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.22628","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T07:53:24Z","timestamp":1696406004000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.22628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,9]]},"references-count":28,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1002\/jor.22628"],"URL":"https:\/\/doi.org\/10.1002\/jor.22628","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,9]]},"assertion":[{"value":"2013-12-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-04-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}