{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T06:00:25Z","timestamp":1785477625439,"version":"3.56.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,9,26]],"date-time":"2014-09-26T00:00:00Z","timestamp":1411689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30872187"],"award-info":[{"award-number":["30872187"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81273006"],"award-info":[{"award-number":["81273006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry","award":["11-01"],"award-info":[{"award-number":["11-01"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Kashin\u2013Beck disease (KBD) is a chronic, deforming endemic osteoarticular disease with altered metabolism of the cartilage matrix. Matrix metalloproteinases (MMPs), aggrecanases (ATAMTSs), and their inhibitors (TIMPs) play important roles in cartilage formation and matrix degradation. This study investigated these proteases and inhibitors in young KBD cartilage. The percentages of chondrocytes staining for MMP\u20101\/\u201013 and MMP\u2010generated DIPEN neoepitope, aggrecanase\u2010generated ITEGE neoepitope in aggrecan in KBD patients were significantly higher than in controls. However, TIMP\u20101 was significantly less numerous than in controls in the superficial and middle zones of KBD samples, the percentage of chondrocytes staining for the TIMP\u20102 was significantly higher than in controls. Staining for MMP\u20101\/\u201013 and, TIMP\u20101\/\u20102 in KBD patients was prominent in the superficial zone and the middle zone of articular cartilage. Staining for ITEGE and DIPEN neoepitopes in KBD samples was prominent in the superficial zone and the middle zone of articular cartilage. The strongest staining for the MMP and aggrecanase\u2010generated neoepitopes was adjacent to areas of chondronecrosis. These results indicated that KBD cartilage destruction depends on collagen\u2010 and aggrecan\u2010degrading proteases such as collagenases (MMP\u20101\/\u201013), as well as aggrecanases. Increased TIMP\u20102 level adjacent to necrotic areas suggest that attempted repair mechanism are also activated. \u00a9 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:47\u201355, 2015.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jor.22708","type":"journal-article","created":{"date-parts":[[2014,9,26]],"date-time":"2014-09-26T16:18:19Z","timestamp":1411748299000},"page":"47-55","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Altered proteolytic activity and expression of MMPs and aggrecanases and their inhibitors in Kashin\u2013Beck disease"],"prefix":"10.1002","volume":"33","author":[{"given":"Jinghong","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Endemic Diseases, Xi'an Jiaotong University College of Medicine, Key Laboratory of Environment and Genes Related to Diseases Ministry of Education Xi'an 710061 Shaanxi P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxiu","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Endemic Diseases, Xi'an Jiaotong University College of Medicine, Key Laboratory of Environment and Genes Related to Diseases Ministry of Education Xi'an 710061 Shaanxi P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Endemic Diseases, Xi'an Jiaotong University College of Medicine, Key Laboratory of Environment and Genes Related to Diseases Ministry of Education Xi'an 710061 Shaanxi P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zentie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Endemic Diseases, Xi'an Jiaotong University College of Medicine, Key Laboratory of Environment and Genes Related to Diseases Ministry of Education Xi'an 710061 Shaanxi P.R. 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