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In this study lateral and medial femoral condyles were respectively collected from patients with late\u2010stage osteoarthritis during the total knee arthroplasty. After digestion of the cartilage tissues with type II collagenase and analysis by fluorescence\u2010activated cell sorting (FACS) with CD146, a chondroprogenitor cell sub\u2010population were isolated and purified. The expression of other MSC\u2010associated markers in the CD146<jats:sup>+<\/jats:sup> chondroprogenitors was analyzed by flow cytometry. Multi\u2010lineage differentiation capacity of CD146<jats:sup>+<\/jats:sup> chondroprogenitors was compared with that of unsorted chondrocytes and adipose\u2010derived MSCs (ADMSCs). Higher percentage of CD146<jats:sup>+<\/jats:sup> chondroprogenitors isolated from the medial femoral condyles was observed than that from the lateral. CD146<jats:sup>+<\/jats:sup> chondroprogenitors expressed high levels of MSC\u2010specific surface antigens, and showed higher chondrogenesis capacity than ADMSCs and unsorted chondrocytes in a 3D cell pellet culture model. Thus CD146 might be a new cell surface marker for cartilage progenitor cell population in the late\u2010stage osteoarthritis. \u00a9 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. 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