{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T01:45:32Z","timestamp":1785030332261,"version":"3.55.0"},"reference-count":18,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,5,21]],"date-time":"2015-05-21T00:00:00Z","timestamp":1432166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institutes of Health and the Aileen Stock Orthopaedic Research Fund","award":["T35-DK007386"],"award-info":[{"award-number":["T35-DK007386"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>We hypothesized that systemic ceftriaxone and high concentration local antibiotics might eradicate peri\u2010implant sepsis. Experiment 1: Eighty\u2010four implants inoculated with biofilm\u2010forming <jats:italic>Staphylococcus aureus<\/jats:italic> were treated in vitro with gentamicin, vancomycin, gentamicin\u2009+\u2009rifampin, or vancomycin\u2009+\u2009rifampin for 2, 4, or 8 days. Experiment 2: Forty\u2010five implants were wired in vivo to rat femurs and inoculated with 1\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> CFU <jats:italic>S. aureus<\/jats:italic>. After 48\u2009h, rats were treated once daily for 5 days with systemic ceftriaxone, local tobramycin or ceftriaxone, and tobramycin. Experiment 3: Forty implants with established <jats:italic>S. aureus<\/jats:italic> biofilms were wired in vivo to rat femurs. After 48\u2009h, rats were treated with systemic ceftriaxone alone or in combination with local gentamicin, gentamicin and rifampin, or vancomycin. Experiment 1: 100% of implants treated in vitro with gentamicin were sterile after 48\u2009h. The other treatments did not become sterile until 4 days. Experiment 2: No implant was culture negative. The combination of systemic ceftriaxone and local tobramycin was significantly better than others (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.008). Experiment 3: Systemic ceftriaxone alone was ineffective. All implants treated with systemic ceftriaxone and local gentamicin were sterile (<jats:italic>p\u2009<\/jats:italic>&lt;\u20090.001), the other groups were less effective. \u00a9 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1320\u20131326, 2015.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jor.22910","type":"journal-article","created":{"date-parts":[[2015,4,16]],"date-time":"2015-04-16T04:37:42Z","timestamp":1429159062000},"page":"1320-1326","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Combined local and systemic antibiotic treatment is effective against experimental <i>Staphylococcus aureus<\/i> peri\u2010implant biofilm infection"],"prefix":"10.1002","volume":"33","author":[{"given":"Anna S.","family":"van der Horst","sequence":"first","affiliation":[{"name":"School of Medicine University of North Carolina Chapel Hill North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suman","family":"Medda","sequence":"additional","affiliation":[{"name":"School of Medicine University of North Carolina Chapel Hill North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ethan","family":"Ledbetter","sequence":"additional","affiliation":[{"name":"School of Medicine University of North Carolina Chapel Hill North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Medicine University of North Carolina Chapel Hill North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Weinhold","sequence":"additional","affiliation":[{"name":"Orthopaedic Research Labs University of North Carolina Chapel Hill North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J.","family":"Del Gaizo","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics University of North Carolina Chapel Hill North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Dahners","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics University of North Carolina Chapel Hill North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.298.6674.648"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(07)70235-9"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4248\/IJOS11022"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.G.01237"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.77B1.7822405"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1381\/0960892041719680"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2011.04.025"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e3181ff2cb1"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"929","DOI":"10.2106\/JBJS.F.00919","article-title":"Locally administered antibiotics for prophylaxis against surgical wound infection. An in vivo study","volume":"89","author":"Yarboro SR","year":"2007","journal-title":"J Bone Joint Surg Am"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.11.044"},{"key":"e_1_2_7_12_1","first-page":"86","article-title":"Biofilm bacteria: Formation and comparative susceptibility to antibiotics","volume":"66","author":"Olson ME","year":"2002","journal-title":"Can J Vet Res"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2012.05288.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00702-13"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.23.6.932"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/17453674.2010.537810"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.21343"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-0266(01)00022-5"},{"key":"e_1_2_7_19_1","first-page":"183","article-title":"The differential activity of aminoglycoside antibiotics with rifampin explored in a kinetic in vitro model of implant\u2010associated infection (Staphylococcus epidermidis)","volume":"10","author":"Richards GK","year":"1994","journal-title":"Adv Perit Dial."}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjor.22910","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjor.22910","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.22910","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T04:13:15Z","timestamp":1696392795000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.22910"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,21]]},"references-count":18,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1002\/jor.22910"],"URL":"https:\/\/doi.org\/10.1002\/jor.22910","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,21]]},"assertion":[{"value":"2015-01-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}