{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T07:07:59Z","timestamp":1763017679901},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,9,16]],"date-time":"2016-09-16T00:00:00Z","timestamp":1473984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) at National Institutes of Health","award":["R01-AR063757","P30-AR057235","R01-AR063757-02S1","R01-AR047867","1K99-AR064837"],"award-info":[{"award-number":["R01-AR063757","P30-AR057235","R01-AR063757-02S1","R01-AR047867","1K99-AR064837"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label \/><jats:p>In this study, we examined early time\u2010dependent changes in articular cartilage and synovium in response to tibial compression and sought the plausible origin of cells that respond to compression in the healer (LGXSM\u20106) and non\u2010healer (LGXSM\u201033) recombinant inbred mouse strains. The right knee of 13\u2010week old male mice was subjected to tibial compression using 9N axial loading. The contralateral left knee served as a control. Knees were harvested at 5, 9, and 14 days post\u2010injury. Histological changes in cartilage and synovium, immunofluorescence pattern of CD44, aggrecan, type\u2010II collagen, cartilage oligomeric matrix protein and the aggrecan neo\u2010epitope NITEGE, and cell apoptosis (by TUNEL) were examined. We used a double nucleoside analog cell\u2010labeling strategy to trace cells responsive to injury. We showed that tibial compression resulted in rupture of anterior cruciate ligament, cartilage matrix loss and chondrocyte apoptosis at the injury site. LGXSM\u201033 showed higher synovitis and ectopic synovial chondrogenesis than LGXSM\u20106 with no differences for articular cartilage lesions. With loading, an altered pattern of CD44 and NITEGE was observed: cells in the impacted area underwent apoptosis, cells closely surrounding the injured area expressed CD44, and cells in the intact area expressed NITEGE. Cells responding to injury were found in the synovium, subchondral bone marrow and the Groove of Ranvier. Taken together, we found no strain differences in chondrocytes in the early response to injury. However, the synovial response was greater in LGXSM\u201033 indicating that, at early time points, there is a genetic difference in synovial cell reaction to injury. \u00a9 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:524\u2013536, 2017.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jor.23413","type":"journal-article","created":{"date-parts":[[2016,9,3]],"date-time":"2016-09-03T13:01:08Z","timestamp":1472907668000},"page":"524-536","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Early changes in the knee of healer and non\u2010healer mice following non\u2010invasive mechanical injury"],"prefix":"10.1002","volume":"35","author":[{"given":"Xin","family":"Duan","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Musculoskeletal Research Center Washington University School of Medicine at Barnes\u2010Jewish Hospital 425 S. 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Euclid Ave. MS 8233 St. Louis Missouri 63110"},{"name":"Department of Biomedical Engineering Washington University in St. Louis at Engineering and Applied Sciences Whitaker Hall, MS 1097 St. Louis Missouri 63130"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew J.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Musculoskeletal Research Center Washington University School of Medicine at Barnes\u2010Jewish Hospital 425 S. Euclid Ave. MS 8233 St. Louis Missouri 63110"},{"name":"Department of Biomedical Engineering Washington University in St. Louis at Engineering and Applied Sciences Whitaker Hall, MS 1097 St. Louis Missouri 63130"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debabrata","family":"Patra","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Musculoskeletal Research Center Washington University School of Medicine at Barnes\u2010Jewish Hospital 425 S. 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