{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T01:46:36Z","timestamp":1793065596882,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,6,6]],"date-time":"2017-06-06T00:00:00Z","timestamp":1496707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["15K10439"],"award-info":[{"award-number":["15K10439"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>The purpose of this study is to investigate the morphometric changes of the subchondral bone during the development of osteoarthritis (OA) in transgenic mice with achondroplasia (<jats:italic>Fgfr3<\/jats:italic><jats:sup>ach<\/jats:sup>) carrying a heterozygous gain\u2010of\u2010function mutation in <jats:italic>Fgfr3<\/jats:italic>. Two OA models (spontaneously developed with age: The aging model, and surgically induced by destabilization of the medial meniscus: The DMM model) were established. Articular cartilage, epiphysis, and metaphysis of the knee joint were histologically and morphometrically compared between wild\u2010type mice, and <jats:italic>Fgfr3<\/jats:italic><jats:sup>ach<\/jats:sup> mice in both OA models. Articular cartilage degeneration was scored according to the Osteoarthritis Research Society International (OARSI) scoring system. Several morphometric parameters including bone mineral density (BMD), bone volume\/tissue volume (BV\/TV), trabecular bone thickness (Tb.Th), and subchondral bone thickness in the medial tibial plateau (MTP) (Sb.Th med) were quantified by micro\u2010computed tomography (CT). In the aging model, although there were no significant differences in the OARSI score between wild\u2010type mice and <jats:italic>Fgfr3<\/jats:italic><jats:sup>ach<\/jats:sup> mice, Sb.Th med and Tb.Th in the epiphysis significantly increased in wild\u2010type mice. In the DMM model, the OARSI score of the medial compartment was significantly lower in <jats:italic>Fgfr3<\/jats:italic><jats:sup>ach<\/jats:sup> mice than in wild\u2010type mice. BMD, BV\/TV, and Tb.Th in the epiphysis increased in wild\u2010type mice and unchanged in <jats:italic>Fgfr3<\/jats:italic><jats:sup>ach<\/jats:sup> mice, and the Sb.Th med was significantly larger in wild\u2010type mice after surgery. Subchondral sclerosis, which preceded the cartilage degeneration, was inhibited in <jats:italic>Fgfr3<\/jats:italic><jats:sup>ach<\/jats:sup> mice. Activated FGFR3 signaling prevented sclerotic changes of the subchondral bone and subsequent cartilage degeneration. \u00a9 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. 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