{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:38:19Z","timestamp":1792679899476,"version":"4.5.0"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,7,31]],"date-time":"2017-07-31T00:00:00Z","timestamp":1501459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007273","name":"Weill Cornell Medical College","doi-asserted-by":"publisher","award":["5 UL1 TR000457\u201009"],"award-info":[{"award-number":["5 UL1 TR000457\u201009"]}],"id":[{"id":"10.13039\/100007273","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5T32OD011000\u201020"],"award-info":[{"award-number":["5T32OD011000\u201020"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["1K08AR068470"],"award-info":[{"award-number":["1K08AR068470"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Mitochondrial (MT) dysfunction is known to occur in chondrocytes isolated from end\u2010stage osteoarthritis (OA) patients, but the role of MT dysfunction in the initiation and early pathogenesis of post\u2010traumatic OA (PTOA) remains unclear. The objective of this study was to investigate chondrocyte MT function immediately following mechanical injury in cartilage, and to determine if the response to injury differed between a weight bearing region (medial femoral condyle; MFC) and a non\u2010weight bearing region (distal patellofemoral groove; PFG) of the same joint. Cartilage was harvested from the MFC and PFG of 10 neonatal bovids, and subjected to injurious compression at varying magnitudes (5\u201017\u2009MPa, 5\u201034\u2009GPa\/s) using a rapid single\u2010impact model. Chondrocyte MT respiratory function, MT membrane polarity, chondrocyte viability, and cell membrane damage were assessed in situ. Cartilage impact resulted in MT depolarization and impaired MT respiratory function within 2\u2009h of injury. Cartilage from a non\u2010weight bearing region of the joint (PFG) was more sensitive to impact\u2010induced MT dysfunction and chondrocyte death than cartilage from a weight\u2010bearing surface (MFC). Our findings suggest that MT dysfunction is an acute response of chondrocytes to cartilage injury, and that MT may play a key mechanobiological role in the initiation and early pathogenesis of PTOA. Clinical significance: Direct therapeutic targeting of MT function in the early post\u2010injury time frame may provide a strategy to block perpetuation of tissue damage and prevent the development of PTOA. \u00a9 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:739\u2013750, 2018.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jor.23651","type":"journal-article","created":{"date-parts":[[2017,7,11]],"date-time":"2017-07-11T11:14:55Z","timestamp":1499771695000},"page":"739-750","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Mitochondrial dysfunction is an acute response of articular chondrocytes to mechanical injury"],"prefix":"10.1002","volume":"36","author":[{"given":"Michelle L.","family":"Delco","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine Cornell University Ithaca New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward D.","family":"Bonnevie","sequence":"additional","affiliation":[{"name":"Sibley School of Mechanical and Aerospace Engineering Cornell University Ithaca New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence J.","family":"Bonassar","sequence":"additional","affiliation":[{"name":"Sibley School of Mechanical and Aerospace Engineering Cornell University Ithaca New York"},{"name":"Nancy E. and Peter C. Meinig School of Biomedical Engineering Cornell University Ithaca New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A.","family":"Fortier","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine Cornell University Ithaca New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11420-011-9251-y"},{"key":"e_1_2_7_3_1","first-page":"285","article-title":"Pathogenetic mechanisms of posttraumatic osteoarthritis: opportunities for early intervention","volume":"4","author":"Kramer WC","year":"2011","journal-title":"Int J Clin Exp Med"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmrj.2012.02.009"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.21359"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947603513487457"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/541230"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00389.2003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201102095"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20110162"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2004.09.010"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/2046-2395-3-6"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.213"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10837"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M800292-MCP200"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200007)43:7<1560::AID-ANR21>3.0.CO;2-S"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2474-14-235"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1002\/art.39460","article-title":"Injurious loading of articular cartilage compromises chondrocyte respiratory function","volume":"68","author":"Coleman MC","year":"2015","journal-title":"Arthritis Rheum"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10237-013-0518-8"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.H.00545"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2009.03.007"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22717"},{"key":"e_1_2_7_23_1","volume-title":"AVMA guidelines for the euthanasia of animals","author":"Leary S","year":"2013"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947603515595071"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947603512455195"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.37782"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025652"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22650"},{"key":"e_1_2_7_29_1","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1002\/jor.23327","article-title":"Time\u2010dependent loss of mitochondrial function precedes progressive histologic cartilage degeneration in a rabbit meniscal destabilization model","volume":"35","author":"Goetz JE","year":"2016","journal-title":"J Orthop Res"},{"key":"e_1_2_7_30_1","doi-asserted-by":"crossref","DOI":"10.1002\/jor.21091","article-title":"Rotenone prevents impact\u2010induced chondrocyte death","author":"Goodwin W","year":"2010","journal-title":"J Orthop Res"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2006.03.012"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20164"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1053\/joca.2001.0482"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90027-7"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(87)90172-8"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23462"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-015-9861-5"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2008.07.008"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2009.05.026"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1999.7197"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2015.02.023"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-8063(97)90124-9"},{"key":"e_1_2_7_43_1","volume-title":"Biologic Joint Reconstruction: alternatives to Arthroplasty","author":"Cole BJ","year":"2009"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11465-014-0306-x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.21552"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2004.08.004"},{"key":"e_1_2_7_47_1","first-page":"201","article-title":"Intracellular mechanics and mechanotransduction associated with chondrocyte deformation during pipette aspiration","volume":"43","author":"Ohashi T","year":"2006","journal-title":"Biorheology"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22303"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2016.01.107"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(70)86307-X"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199608)168:2<424::AID-JCP21>3.0.CO;2-1"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2942"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellbi.2007.02.002"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.20675"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1053\/joca.1999.0314"},{"key":"e_1_2_7_56_1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1002\/jcp.20491","article-title":"Rate of oxygen consumption by isolated articular chondrocytes is sensitive to medium glucose concentration","volume":"206","author":"Heywood HK","year":"2005","journal-title":"J Cell Physiol"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.11438"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2012.07.035"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.4A6511.2005"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjor.23651","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.23651","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T02:10:39Z","timestamp":1694571039000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.23651"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,31]]},"references-count":58,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1002\/jor.23651"],"URL":"https:\/\/doi.org\/10.1002\/jor.23651","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,31]]},"assertion":[{"value":"2016-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-06-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-07-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}