{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T15:24:57Z","timestamp":1780068297327,"version":"3.54.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,11,28]],"date-time":"2017-11-28T00:00:00Z","timestamp":1511827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"UHealth Sports Medicine Institute"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Traumatic knee injuries often result in damage to articular cartilage and other joint structures. Such trauma is a strong risk factor for the future development and progression of osteoarthritis (OA). The molecular mechanisms and signaling pathways modulating response to knee joint trauma remain unclear. Moreover, investigations of biomarkers influencing responses have been targeted rather than broad, unbiased discovery studies. Herein, we characterize the complete complement of extracellular RNA (exRNA) in the synovial fluid of 14 subjects following knee injury. Fluid was collected during surgery from the injured knees, and from the contralateral knee in a subset, undergoing surgical repair of the ACL and\/or meniscal repair\/debridement. Arthroscopic grading of chondral damage in four knee compartments was performed using the Outerbridge classification. exRNA was extracted and subjected to massively parallel total RNA sequencing. Differential abundance of RNA was calculated between the subject cohorts of injured and non\u2010injured knee, average Outerbridge score \u22650.5 and less, and chronic and acute injury duration defined as \u22644 months till surgery or longer. Overall, expression of several thousand genes was identified in the synovial fluid. Furthermore, differential expression analysis suggests a role of exRNA fragments of matrix metalloproteinases and skeletal muscle fiber genes in the response to traumatic injury. Together, these data suggest that high\u2010throughput approaches can indicate exRNA molecular signatures following knee trauma. Future studies are required to more fully characterize the biological roles of these exRNA and the cadence of their respective release that may lead to translational treatment options for post\u2010traumatic OA. \u00a9 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1659\u20131665, 2018.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jor.23802","type":"journal-article","created":{"date-parts":[[2017,11,6]],"date-time":"2017-11-06T13:42:37Z","timestamp":1509975757000},"page":"1659-1665","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Transcriptomic analysis of synovial extracellular RNA following knee trauma: A pilot study"],"prefix":"10.1002","volume":"36","author":[{"given":"Anthony J.","family":"Griswold","sequence":"first","affiliation":[{"name":"UHealth Sports Medicine Institute Miller School of Medicine University of Miami Miami Florida"},{"name":"John P. 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Hussman Institute for Human Genomics Miller School of Medicine University of Miami Miami Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyong","family":"Wang","sequence":"additional","affiliation":[{"name":"UHealth Sports Medicine Institute Miller School of Medicine University of Miami Miami Florida"},{"name":"John P. Hussman Institute for Human Genomics Miller School of Medicine University of Miami Miami Florida"},{"name":"Dr. John T. Macdonald Foundation Department of Human Genetics Miller School Medicine University of Miami Miami Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danica D.","family":"Vance","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Columbia University Medical Center New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayley","family":"Ennis","sequence":"additional","affiliation":[{"name":"UHealth Sports Medicine Institute Miller School of Medicine University of Miami Miami Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marvin K.","family":"Smith","sequence":"additional","affiliation":[{"name":"UHealth Sports Medicine Institute Miller School of Medicine University of Miami Miami Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Best","sequence":"additional","affiliation":[{"name":"UHealth Sports Medicine Institute Miller School of Medicine University of Miami Miami Florida"},{"name":"Departments of Orthopedics, Biomedical Engineering, Kinesiology Miller School Medicine University of Miami Miami Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffery M.","family":"Vance","sequence":"additional","affiliation":[{"name":"UHealth Sports Medicine Institute Miller School of Medicine University of Miami Miami Florida"},{"name":"John P. Hussman Institute for Human Genomics Miller School of Medicine University of Miami Miami Florida"},{"name":"Dr. John T. Macdonald Foundation Department of Human Genetics Miller School Medicine University of Miami Miami Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret A.","family":"Pericak\u2010Vance","sequence":"additional","affiliation":[{"name":"UHealth Sports Medicine Institute Miller School of Medicine University of Miami Miami Florida"},{"name":"John P. Hussman Institute for Human Genomics Miller School of Medicine University of Miami Miami Florida"},{"name":"Dr. John T. Macdonald Foundation Department of Human Genetics Miller School Medicine University of Miami Miami Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee D.","family":"Kaplan","sequence":"additional","affiliation":[{"name":"UHealth Sports Medicine Institute Miller School of Medicine University of Miami Miami Florida"},{"name":"Departments of Orthopedics, Biomedical Engineering, Kinesiology Miller School Medicine University of Miami Miami Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.23176"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2013.07.003"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-200704000-00012"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2009.08.010"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2011.07.015"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546514526139"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/acr.22359"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csm.2012.08.017"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2012.02.012"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.33445"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v4.27494"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1677\/JME-07-0160"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v4.26760"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-015-0284-5"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carpath.2015.04.007"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1212\/NXI.0000000000000267"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1501580"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546516664889"},{"key":"e_1_2_7_20_1","first-page":"1","article-title":"Gene expression in human meniscal tears has limited association with early degenerative changes in knee articular cartilage","author":"Brophy RH","year":"2016","journal-title":"Connect Tissue Res"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2016.04.019"},{"key":"e_1_2_7_22_1","first-page":"1","article-title":"Dynamic gene expression profile changes in synovial fluid following meniscal injury; osteoarthritis (OA) markers found","volume":"1","author":"Vance DD","year":"2014","journal-title":"J Exerc Sports Orthop"},{"key":"e_1_2_7_23_1","unstructured":"AndrewsS.2010. 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