{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:43:23Z","timestamp":1790095403032,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,11,30]],"date-time":"2017-11-30T00:00:00Z","timestamp":1512000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1U54GM104942\u201001"],"award-info":[{"award-number":["1U54GM104942\u201001"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Adjuvant treatments including Betadine, Dakin's solution (sodium hypochlorite), or hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) have been attempted to eradicate prosthetic joint infection caused by biofilm or intracellular bacteria. The purpose of this study was to evaluate the in vitro abilities of chemical adjuvants to decrease <jats:italic>Staphylococcus aureus<\/jats:italic> (<jats:italic>S. aureus<\/jats:italic>) biofilm presence on orthopaedic implant grade materials, including titanium, stainless steel, and cobalt chrome. <jats:italic>S. aureus<\/jats:italic> biofilms were grown for 48\u2009h and evaluated for baseline colony forming units\/centimeter squared (CFU\/cm<jats:sup>2<\/jats:sup>) and compared to treatments with Betadine, Dakin's solution, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, or 1% chlorine dioxide (ClO<jats:sub>2<\/jats:sub>). Control discs (<jats:italic>n<\/jats:italic>\u2009=\u200918) across all metals had an average of 4.2\u2009\u00d7\u200910<jats:sup>7<\/jats:sup> CFU\/cm<jats:sup>2<\/jats:sup>. All treatments had statistically significant reductions in CFU\/cm<jats:sup>2<\/jats:sup> when compared to respective control discs (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). For all metals combined, the most efficacious treatments were Betadine and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, with an average 98% and 97% CFU\/cm<jats:sup>2<\/jats:sup> reduction, respectively. There were no significant differences between reductions seen with Betadine and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, but both groups had statistically greater reductions than Dakin's solution and ClO<jats:sub>2<\/jats:sub>. There was no change in antibiotic resistance patterns after treatment. Analysis of <jats:italic>S. aureus<\/jats:italic> biofilms demonstrated a statistically significant reduction in biofilm after a five\u2010minute treatment with the modalities, with an average two log reduction in CFU\/cm<jats:sup>2<\/jats:sup>. Statement of clinical significance: While statistically significant reductions in CFU\/cm<jats:sup>2<\/jats:sup> were accomplished with chemical adjuvant treatments, the overall concentration of bacteria never fell below 10<jats:sup>5<\/jats:sup> CFU\/cm<jats:sup>2<\/jats:sup>, leading to questionable clinical significance. Further techniques to eradicate biofilm should be investigated. \u00a9 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1599\u20131604, 2018.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jor.23804","type":"journal-article","created":{"date-parts":[[2017,11,15]],"date-time":"2017-11-15T12:19:02Z","timestamp":1510748342000},"page":"1599-1604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Topical adjuvants incompletely remove adherent <i>Staphylococcus aureus<\/i> from implant materials"],"prefix":"10.1002","volume":"36","author":[{"given":"Emily P.","family":"Ernest","sequence":"first","affiliation":[{"name":"Robert C. Byrd Health Sciences Center Department of Orthopaedics West Virginia University School of Medicine P.O. Box 9196 Morgantown West Virginia 26506\u20109196"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony S.","family":"Machi","sequence":"additional","affiliation":[{"name":"Robert C. Byrd Health Sciences Center West Virginia University School of Medicine P.O. 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