{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T12:53:53Z","timestamp":1793969633701,"version":"4.8.0"},"reference-count":69,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,3,14]],"date-time":"2018-03-14T00:00:00Z","timestamp":1520985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["5T32OD011000\u201020"],"award-info":[{"award-number":["5T32OD011000\u201020"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["1K08AR068470"],"award-info":[{"award-number":["1K08AR068470"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["5 UL1 TR000457\u201009"],"award-info":[{"award-number":["5 UL1 TR000457\u201009"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>No disease\u2010modifying osteoarthritis (OA) drugs are available to prevent posttraumatic osteoarthritis (PTOA). Mitochondria (MT) mediate the pathogenesis of many degenerative diseases, and recent evidence indicates that MT dysfunction is a peracute (within minutes to hours) response of cartilage to mechanical injury. The goal of this study was to investigate cardiolipin\u2010targeted mitoprotection as a new strategy to prevent chondrocyte death and cartilage degeneration after injury. Cartilage was harvested from bovine knee joints and subjected to a single, rapid impact injury (24.0\u2009\u00b11.4\u2009MPa, 53.8\u2009\u00b1\u20095.3\u2009GPa\/s). Explants were then treated with a mitoprotective peptide, SS\u201031 (1\u00b5M), immediately post\u2010impact, or at 1, 6, or 12\u2009h after injury, and then cultured for up to 7 days. Chondrocyte viability and apoptosis were quantified in situ using confocal microscopy. Cell membrane damage (lactate dehydrogenase activity) and cartilage matrix degradation (glycosaminoglycan loss) were quantified in cartilage\u2010conditioned media. SS\u201031 treatment at all time points after impact resulted in chondrocyte viability similar to that of un\u2010injured controls. This effect was sustained for up to a week in culture. Further, SS\u201031 prevented impact\u2010induced chondrocyte apoptosis, cell membrane damage, and cartilage matrix degeneration. Clinical Significance: This study is the first investigation of cardiolipin\u2010targeted mitoprotective therapy in cartilage. These results suggest that even when treatment is delayed by up to 12\u2009h after injury, mitoprotection may be a useful strategy in the prevention of PTOA. \u00a9 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2147\u20132156, 2018.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jor.23882","type":"journal-article","created":{"date-parts":[[2018,2,22]],"date-time":"2018-02-22T05:56:43Z","timestamp":1519279003000},"page":"2147-2156","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Mitoprotective therapy preserves chondrocyte viability and prevents cartilage degeneration in an ex vivo model of posttraumatic osteoarthritis"],"prefix":"10.1002","volume":"36","author":[{"given":"Michelle L.","family":"Delco","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine Cornell University Ithaca New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward D.","family":"Bonnevie","sequence":"additional","affiliation":[{"name":"Sibley School of Mechanical and Aerospace Engineering Cornell University Ithaca New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hazel S.","family":"Szeto","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Weill Cornell Medical College New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence J.","family":"Bonassar","sequence":"additional","affiliation":[{"name":"Sibley School of Mechanical and Aerospace Engineering Cornell University Ithaca New York"},{"name":"Nancy E. and Peter C. 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