{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T18:13:52Z","timestamp":1780337632654,"version":"3.54.1"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T00:00:00Z","timestamp":1549929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Ministry of Health of the Czech Republic, Department Program for Research and Development","award":["17-31564A"],"award-info":[{"award-number":["17-31564A"]}]},{"name":"Charles University in Prague, project GA UK","award":["n. 336218"],"award-info":[{"award-number":["n. 336218"]}]},{"name":"Charles University in Prague, Specific University Research","award":["RVO:67985823"],"award-info":[{"award-number":["RVO:67985823"]}]},{"name":"Charles University in Prague, Specific University Research","award":["n. SVV 260440"],"award-info":[{"award-number":["n. SVV 260440"]}]},{"name":"The KZCR, a.s., Czech Republic, IGP KZ","award":["n. 217116002"],"award-info":[{"award-number":["n. 217116002"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Idiopathic <jats:italic>pes equinovarus<\/jats:italic> (clubfoot) is a congenital deformity of the feet and lower legs. Clubfoot belongs to a group of fibro\u2010proliferative disorders but its origin remains unknown. Our study aimed to achieve the first complex proteomic comparison of clubfoot contracted tissue of the foot (medial side; <jats:italic>n<\/jats:italic>\u2009=\u200916), with non\u2010contracted tissue (lateral side; <jats:italic>n<\/jats:italic>\u2009=\u200913). We used label\u2010free mass spectrometry quantification and immunohistochemistry. Seven proteins were observed to be significantly upregulated in the medial side (asporin, collagen type III, V, and VI, versican, tenascin\u2010C, and transforming growth factor beta induced protein) and four in the lateral side (collagen types XII and XIV, fibromodulin, and cartilage intermediate layer protein 2) of the clubfoot. Comparison of control samples from cadavers brought only two different protein concentrations (collagen types I and VI). We also revealed pathological calcification and intracellular positivity of transforming growth factor beta only in the contracted tissue of clubfoot. Most of the 11 differently expressed proteins are strongly related to the extracellular matrix architecture and we assume that they may play specific roles in the pathogenesis of this deformity. These proteins seem to be promising targets for future investigations and treatment of this disease. \u00a9 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. 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