{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:35:06Z","timestamp":1790235306808,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T00:00:00Z","timestamp":1549929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"crossref","award":["DFG, SFB\/TR 79"],"award-info":[{"award-number":["DFG, SFB\/TR 79"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University Hospital Carl Gustav Carus at Technische Universit\u00e4t Dresden","award":["MedDrive Grant 60.373"],"award-info":[{"award-number":["MedDrive Grant 60.373"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Adult stem cells are a promising tool to positively influence bone regeneration. Concentrated bone marrow therapy entails isolating osteoprogenitor cells during surgery with, however, only low cells yield. Two step stem cell therapy requires an additional harvesting procedure but generates high numbers of progenitor cells that facilitate osteogenic pre\u2010differentiation. To further improve bone regeneration, stem cell therapy can be combined with growth factors from platelet rich plasma (PRP) or its lysate (PL) to potentially fostering vascularization. The aim of this study was to investigate the effects of bone marrow concentrate (BMC), osteogenic pre\u2010differentiation of mesenchymal stromal cells (MSCs), and PL on bone regeneration and vascularization. Bone marrow from four different healthy human donors was used for either generation of BMC or for isolation of MSCs. Seventy\u2010two mice were randomized to six groups (Control, PL, BMC, BMC\u2009+\u2009PL, pre\u2010differentiated MSCs, pre\u2010differentiated MSCs\u2009+\u2009PL). The influence of PL, BMC, and pre\u2010differentiated MSCs was investigated systematically in a 2\u2009mm femoral bone defect model. After a 6\u2010week follow\u2010up, the pre\u2010differentiated MSCs\u2009+\u2009PL group showed the highest bone volume, highest grade of histological defect healing and highest number of bridged defects with measurable biomechanical stiffness. Using expanded and osteogenically pre\u2010differentiated MSCs for treatment of a critical\u2010size bone defect was favorable with regards to bone regeneration compared to treatment with cells from BMC. The addition of PL alone had no significant influence; therefore the role of PL for bone regeneration remains unclear. \u00a9 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. 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