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Disuse models report macroscopic bone loss due to muscle inactivity and immobilization, yet only recently has there been quantification of the effects of disuse on the vascular pores and osteocyte network, which are believed to play an important role in mechanotransduction via interstitial fluid flow. The goal of this study was to perform a high\u2010resolution analysis of the effects of muscle inactivity on intracortical porosity and osteocyte lacunar density in skeletally mature rats. Muscle paralysis was induced in 20\u2010week\u2010old female Sprague Dawley rats by injection of botulinum neurotoxin. Rats were injected in the right hindlimb muscles with either Botox (BTX, <jats:italic>n<\/jats:italic>\u2009=\u20098) or saline solution (CTRL, <jats:italic>n<\/jats:italic>\u2009=\u20098), with a third group used as baseline controls (<jats:italic>n<\/jats:italic>\u2009=\u20098). Four weeks after injection, Botox caused a \u223c60% reduction in hindlimb muscle mass. High\u2010resolution micro\u2010CT analysis showed that Botox\u2010induced muscle paralysis increased vascular canal porosity and reduced osteocyte lacunar density within the tibial metaphysis cortex. Cortical thickness and other areal properties were diminished in the proximal tibial metaphysis, whereas no differences were found in the mid\u2010diaphysis. Within the BTX group, the injected limbs showed a lower cancellous bone volume fraction relative to the contralateral limb. These results indicate that diminished muscle activity alters the vascular canal porosity and osteocyte lacunar density in cortical bone, which could alter interstitial fluid flow, affecting molecular transport and the transmission of mechanical signals to osteocytes. \u00a9 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. 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