{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T22:23:36Z","timestamp":1778711016529,"version":"3.51.4"},"reference-count":61,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,5,17]],"date-time":"2019-05-17T00:00:00Z","timestamp":1558051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["AR49718"],"award-info":[{"award-number":["AR49718"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Cryopreserved bone allografts (CBA) used to reconstruct segmental bone defects provide immediate structural stability, but are vulnerable to infection, non\u2010union and late stress fracture as the majority of the allograft remains largely avascular. We sought to improve the bone vascularity and bone formation of CBAs by surgical angiogenesis with an implanted arteriovenous (AV) bundle, using a porcine tibial defect model. Cryopreserved tibial bone allografts were transplanted in swine leukocyte antigen (SLA) mismatched Yucatan minipigs to reconstruct a 3.5\u2009cm segmental tibial defect. A cranial tibial AV\u2010bundle was placed within its intramedullary canal to induce angiogenesis. The AV bundle was patent in eight pigs and ligated in a control group of eight pigs. At 20 weeks neo\u2010angiogenesis was evaluated by micro\u2010angiography. Bone formation was measured by quantitative histomorphometry and micro\u2010computed tomography. Seven of eight AV\u2010bundles in the revascularized group were patent. One had thrombosed due to allograft displacement. Total vascular volume was higher in the revascularized allografts compared to the ligated group (<jats:italic>p<\/jats:italic>\u2009=\u20090.015). Revascularized allografts had increased levels of bone formation on the allograft endosteal surface compared to the ligated control group (<jats:italic>p<\/jats:italic>\u2009=\u20090.05). Surgical angiogenesis of porcine tibial CBAs by intramedullary implantation of an AV\u2010bundle creates an enhanced autogenous neoangiogenic circulation and accelerates active bone formation on allograft endosteal surfaces. \u00a9 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:1698\u20131708, 2019<\/jats:p>","DOI":"10.1002\/jor.24318","type":"journal-article","created":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T15:28:44Z","timestamp":1556724524000},"page":"1698-1708","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of Surgical Angiogenesis on Segmental Bone Reconstruction With Cryopreserved Massive\u2010Structural Allografts in a Porcine Tibia Model"],"prefix":"10.1002","volume":"37","author":[{"given":"Noortje J.","family":"Visser","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery Microvascular Research Laboratory, Mayo Clinic 200 First Street SW Rochester Minnesota"},{"name":"Department of Plastic and Reconstructive Surgery Amsterdam UMC, University of Amsterdam Amsterdam Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa 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Surgery University of Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander Y.","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Microvascular Research Laboratory, Mayo Clinic 200 First Street SW Rochester Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allen T.","family":"Bishop","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Microvascular Research Laboratory, Mayo Clinic 200 First Street SW Rochester 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