{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:58:26Z","timestamp":1792123106701,"version":"4.4.0"},"reference-count":45,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008459","name":"University of Calgary","doi-asserted-by":"publisher","award":["Cumming School of Medicine Postdoctoral Scholarshi"],"award-info":[{"award-number":["Cumming School of Medicine Postdoctoral Scholarshi"]}],"id":[{"id":"10.13039\/100008459","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102014\u201004586"],"award-info":[{"award-number":["RGPIN\u20102014\u201004586"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Proteoglycan 4 (PRG4) is a mucin\u2010like glycoprotein important for joint health. Mice lacking <jats:italic>Prg4<\/jats:italic> demonstrate degeneration of the cartilage and altered skeletal morphology. The purpose of this study was to examine if <jats:italic>Prg4<\/jats:italic> deficiency leads to subchondral bone defects and if these defects could be mitigated through intra\u2010articular injection of recombinant human PRG4 (rhPRG4). Mice deficient in <jats:italic>Prg4<\/jats:italic> expression demonstrated increased cartilage thickness and increased subchondral bone porosity compared with C57BL\/6 controls. While the porosity of the subchondral bone of <jats:italic>Prg4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice decreased over time with maturation, intra\u2010articular injection of rhPRG4 was able to forestall the increase in porosity. In contrast, neither hyaluronan (HA) nor methylprednisolone injections had beneficial effects on the subchondral bone porosity in the <jats:italic>Prg4<\/jats:italic> knockout mice. Bone marrow progenitor cells from <jats:italic>Prg4<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice demonstrated reduced osteogenic differentiation capacity at 4 weeks of age, but not at 16 weeks of age. While most studies on PRG4\/lubricin focus on the health of the cartilage, this study demonstrates that PRG4 plays a role in the maturation of the subchondral bone. Furthermore, increasing joint lubrication\/viscosupplementation through injection of HA or controlling joint inflammation through injection of methylprednisolone may help maintain the cartilage surface, but had no positive effect on the subchondral bone in animals lacking <jats:italic>Prg4<\/jats:italic>. Therefore, alterations in the subchondral bone in models with absent or diminished <jats:italic>Prg4<\/jats:italic> expression should not be overlooked when investigating changes within the articular cartilage regarding the pathogenesis of osteoarthritis\/arthrosis. \u00a9 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. 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