{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T04:10:01Z","timestamp":1778645401096,"version":"3.51.4"},"reference-count":17,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T00:00:00Z","timestamp":1581379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1106982"],"award-info":[{"award-number":["APP1106982"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Infection of orthopedic implants is a growing clinical challenge to manage due to the proliferation of drug\u2010resistant bacterial strains. In this study, we aimed to investigate whether the treatment of implants with ceragenin\u201090 (CSA\u201090), a synthetic compound based on endogenous antibacterial peptides, could prevent infection in a novel rat model of periprosthetic joint infection (PJI) challenged with either local or systemic <jats:italic>Staphylococcus aureus<\/jats:italic>. A novel preclinical model of PJI was created using press\u2010fit porous titanium implants in the distal femur of male Wistar rats. Sterile implants were pre\u2010treated with 500\u2009\u03bcg CSA\u201090 in saline. <jats:italic>S. aureus<\/jats:italic> was applied either directly at the time of surgery or administered via tail vein injection immediately afterward. Animals were monitored daily for clinical and radiographic evidence of infection for a total of 6 weeks. Post\u2010study microbiological, radiographic, and histological analysis were performed to determine the incidence of PJI and assess osseointegration. CSA\u201090 treated groups demonstrated a reduced rate of PJI as confirmed by deep tissue swab culture at the time of cull compared with untreated groups with both local (33% vs 100%; <jats:italic>P<\/jats:italic>\u2009=\u2009.009) and systemic (10% vs 90%; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.0001) <jats:italic>S. aureus<\/jats:italic> inoculation. Median survival time also increased from 8 to 17 days and from 8 to 42 days, respectively. In conclusion, this study describes a novel preclinical model of local and hematogenous PJI using a porous metal implant. CSA\u201090 reduced the incidence of PJI in this model supporting its further development as an antimicrobial coating for orthopedic implants.<\/jats:p>","DOI":"10.1002\/jor.24618","type":"journal-article","created":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T08:53:34Z","timestamp":1580720014000},"page":"2065-2073","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["CSA\u201090 reduces periprosthetic joint infection in a novel rat model challenged with local and systemic <i>Staphylococcus aureus<\/i>"],"prefix":"10.1002","volume":"38","author":[{"given":"Rebecca J.","family":"Mills","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Research and Biotechnology The Children's Hospital at Westmead Westmead New South Wales Australia"},{"name":"Division of Medical Sciences University of Oxford Oxford 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