{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T15:45:51Z","timestamp":1774280751002,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T00:00:00Z","timestamp":1582675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transforming growth factor \u03b21(TGF\u2010\u03b21)\/Smad3 pathway promotes the pathological progression of subchondral bone in osteoarthritis. The aim of this study is to determine the effect of low\u2010intensity pulsed ultrasound (LIPUS) on the pathological progression and TGF\u2010\u03b21\/Smad3 pathway of subchondral bone in temporomandibular joint osteoarthritis (TMJOA). Rabbit TMJOA model was established by type II collagenase induction. The left joint in this model was continuously stimulated with LIPUS for 3 and 6 weeks (1\u2009MHz; 30\u2009mW\/cm<jats:sup>2<\/jats:sup>) for 20\u2009min\/day. The morphological and histological features of subchondral bone were respectively examined by microcomputed tomography and Safranin\u2010O staining. The number of osteoclasts was quantitatively assessed by tartrate\u2010resistant acid phosphatase staining. Immunohistochemistry and Western blot analysis were conducted to evaluate the protein expression of Cathepsin K and TGF\u2010\u03b21\/Smad3 pathway. The results indicated that LIPUS could improve the trabecular microstructure and histological characteristics of subchondral bone in rabbit TMJOA. It also suppressed abnormal subchondral bone resorption and activation of TGF\u2010\u03b21\/Smad3 pathway, characterized by the number of osteoclasts, protein expression levels of Cathepsin K, TGF\u2010\u03b21, type II TGF\u03b2 receptor, and phosphorylated Smad3 (pSmad3) were decreased. In conclusion, LIPUS promoted the quality of subchondral bone by suppressing osteoclast activity and TGF\u2010\u03b21\/Smad3 pathway in rabbit TMJOA.<\/jats:p>","DOI":"10.1002\/jor.24628","type":"journal-article","created":{"date-parts":[[2020,2,15]],"date-time":"2020-02-15T08:09:25Z","timestamp":1581754165000},"page":"2505-2512","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Low\u2010intensity pulsed ultrasound protects subchondral bone in rabbit temporomandibular joint osteoarthritis by suppressing TGF\u2010\u03b21\/Smad3 pathway"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6015-3573","authenticated-orcid":false,"given":"Xin","family":"Yi","sequence":"first","affiliation":[{"name":"Department of Oral Anatomy and Physiology School of Stomatology, China Medical University  Shenyang 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