{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T22:25:42Z","timestamp":1793744742904,"version":"4.6.1"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T00:00:00Z","timestamp":1586908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Eicosapentanoic acid (EPA) is an antioxidant and omega\u20103 polyunsaturated fatty acid that reduces inflammatory cytokine production. Gelatin hydrogel can be used as a carrier of a physiologically active substance that release it gradually for an average of ~3 weeks. Therefore, this study aimed to clarify the effect of EPA\u2010incorporating gelatin hydrogels on osteoarthritis (OA) progression in vivo. Ten\u2010week\u2010old male C57BL\/6J mice were randomly divided into six groups (<jats:italic>n<\/jats:italic>\u2009=\u20096): Sham, destabilization of the medial meniscus (DMM), Corn: DMM\u2009+\u20092\u2009\u00b5L corn oil, EPA injection alone (EPA\u2010I): DMM\u2009+\u20092\u2009\u00b5L corn oil\u2009+\u2009125\u2009\u03bcg\/\u03bcL EPA, Gel: DMM\u2009+\u2009gelatin hydrogels, and EPA\u2010G: DMM\u2009+\u2009125\u2009\u03bcg\/\u03bcL EPA\u2010incorporating gelatin hydrogels. The mice were euthanized at 8 weeks after DMM or Sham surgery, and subjected to histological evaluation. Matrix\u2010metalloproteinases\u20103 (MMP\u20103), MMP\u201013, interleukin\u20101\u03b2 (IL\u20101\u03b2), p\u2010IKK \u03b1\/\u03b2, CD86, and CD163 protein expression in the synovial cartilage was detected by immunohistochemical staining. F4\/80 expression was also assessed using the F4\/80 score of macrophage. Histological score was significantly lower in EPA\u2010G than in EPA\u2010I. MMP\u20103\u2010, MMP\u201013\u2010, IL\u20101\u03b2\u2010, and p\u2010IKK \u03b1\/\u03b2\u2010positive cell ratio was significantly lower in EPA\u2010G than in EPA\u2010I. However, CD86\u2010 and CD163\u2010positive cell ratio was not significantly different between EPA\u2010I and EPA\u2010G. The average\u2010sum F4\/80 score of macrophage in EPA\u2010G was significantly lower than that in EPA\u2010I. EPA\u2010incorporating gelatin hydrogels were shown to prevent OA progression in vivo more effectively than EPA injection alone. Our results suggested that intra\u2010articular administration of controlled\u2010release EPA can be a new therapeutic approach for treating OA.<\/jats:p>","DOI":"10.1002\/jor.24688","type":"journal-article","created":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T11:11:41Z","timestamp":1586430701000},"page":"2157-2169","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Gelatin hydrogels with eicosapentaenoic acid can prevent osteoarthritis progression in vivo in a mouse model"],"prefix":"10.1002","volume":"38","author":[{"given":"Masanori","family":"Tsubosaka","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Kobe University School of Medicine  Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinsuke","family":"Kihara","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Kobe University School of Medicine  Kobe 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