{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T15:15:27Z","timestamp":1783610127852,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T00:00:00Z","timestamp":1595203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Many surgeons continue to face the clinical dilemma of interpreting a positive aspiration or unexpected positive <jats:italic>Cutibacterium acnes (C. acnes)<\/jats:italic> culture. There are factors that complicate the interpretation of positive cultures including variations in both frequency of false positive cultures and virulence properties. As indices of virulence, hemolytic strains, from previously confirmed clinically infected shoulders, were compared with non\u2010hemolytic isolates determined to be contaminants, by RNA\u2010sequencing (RNA\u2010Seq). Six <jats:italic>C. acnes<\/jats:italic> isolates from patients who underwent revision total shoulder arthroplasty (TSA) were identified based on previously described infection criteria. Three <jats:italic>C. acnes<\/jats:italic> isolates from each group underwent RNA\u2010Seq. Differential gene expression analysis, principal component analysis (PCA), and heatmap analysis were used to determine the gene variation and patterning between the definite infection and probable contaminant isolates. Differential gene expression analysis identified genes that were differentially expressed between the isolates classified as definite infection and isolates classified as probable contaminants. PCA using a 500 gene subset of identified genes was able to find combinations of these genes that separated out the definite infection and probable contaminants isolates. The heatmap demonstrated similar gene expression in the three Definite Infections isolates, and significantly different expression when compared with the probable contaminant isolates. Clinical significance: <jats:italic>C. acnes<\/jats:italic> revision TSA isolates classified as definite infection and probable contaminant demonstrated a similar gene expression pattern to each respective group and different gene expression pattern when compared between groups. These findings indicate distinct differences in <jats:italic>C. acnes<\/jats:italic> strains associated with clinically relevant orthopedic TSA infections.<\/jats:p>","DOI":"10.1002\/jor.24798","type":"journal-article","created":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T15:34:24Z","timestamp":1594308864000},"page":"2731-2739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Pathogenic genetic variations of <i>C. acnes<\/i> are associated with clinically relevant orthopedic shoulder infections"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1327-0379","authenticated-orcid":false,"given":"K. Keely","family":"Boyle","sequence":"first","affiliation":[{"name":"Department of Orthopaedics State University of New York  Buffalo New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3547-1182","authenticated-orcid":false,"given":"Brandon J.","family":"Marzullo","sequence":"additional","affiliation":[{"name":"Genomics and Bioinformatics Core, NYS Center of Excellence in Bioinformatics and Life Sciences (CBLS) State University of New York  Buffalo New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald A.","family":"Yergeau","sequence":"additional","affiliation":[{"name":"Genomics and Bioinformatics Core, NYS Center of Excellence in Bioinformatics and Life Sciences (CBLS) State University of New York  Buffalo New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott R.","family":"Nodzo","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics State University of New York  Buffalo New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John K.","family":"Crane","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease State University of New York  Buffalo New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R.","family":"Duquin","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics State University of New York  Buffalo New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-014-3696-5"},{"key":"e_1_2_7_3_1","first-page":"E93","article-title":"Hemolysis as a clinical marker for propionibacterium acnes orthopedic infection","volume":"43","author":"Nodzo SR","year":"2014","journal-title":"Am J Orthop"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00092-13"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.1.326-334.2005"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jse.2012.07.007"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jse.2011.08.052"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.12.023"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000725"},{"key":"e_1_2_7_10_1","first-page":"4","article-title":"Analysis of the in vivo transcriptome of Bordetella pertussis during infection of mice","author":"Wong TY","year":"2019","journal-title":"mSphere"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-184"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0204542"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026905"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0142375"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0200583"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111988"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.5435\/JAAOS-D-17-00394"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jse.2014.12.021"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.RVW.M.00055"},{"key":"e_1_2_7_20_1","first-page":"39","article-title":"Propionibacterium acnes susceptibility and correlation with hemolytic phenotype","volume":"9","author":"Wright TE","year":"2016","journal-title":"Infect Dis"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014797"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.N.00104"},{"key":"e_1_2_7_23_1","first-page":"803","article-title":"The microbial ecology of pilosebaceous units isolated from human skin","volume":"130","author":"Leeming JP","year":"1984","journal-title":"J Gen Microbiol"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/16.Supplement_4.S175"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1100330"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2016.02.025"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11420-016-9528-2"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00450-11"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11420-013-9366-4"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00834-12"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.blo.0000218727.14097.d5"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jse.2016.11.054"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.17.00434"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.01.024"},{"key":"e_1_2_7_35_1","first-page":"205","article-title":"Infections from biomaterials and implants: a race for the surface","volume":"14","author":"Gristina AG","year":"1988","journal-title":"Med Prog Technol"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(94)35179-1"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1945.30"},{"key":"e_1_2_7_38_1","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1128\/jcm.8.2.238-241.1978","article-title":"Synergistic lysis of erythrocytes by Propionibacterium acnes","volume":"8","author":"Choudhury TK","year":"1978","journal-title":"J Clin Microbiol"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00463-13"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jse.2017.12.025"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.73.011303.073626"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1418"},{"key":"e_1_2_7_43_1","doi-asserted-by":"crossref","first-page":"20384","DOI":"10.1016\/S0021-9258(17)30515-X","article-title":"Active\u2010site residues of 2\u2010keto\u20104\u2010hydroxyglutarate aldolase from Escherichia coli. Bromopyruvate inactivation and labeling of glutamate 45","volume":"265","author":"Vlahos CJ","year":"1990","journal-title":"J Biol Chem"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjor.24798","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.24798","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jor.24798","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.24798","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T19:30:32Z","timestamp":1693855832000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.24798"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,20]]},"references-count":42,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1002\/jor.24798"],"URL":"https:\/\/doi.org\/10.1002\/jor.24798","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,20]]},"assertion":[{"value":"2020-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}