{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T09:00:08Z","timestamp":1784106008361,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T00:00:00Z","timestamp":1597017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fracture\u2010related infection (FRI) is a serious complication following musculoskeletal trauma. Accurate diagnosis and appropriate treatment depend on retrieving adequate deep tissue biopsies for bacterial culture. The aim of this cohort study was to compare intraoperative tissue cultures obtained by the Reamer\u2010Irrigator\u2010Aspirator system (RIA)\u2010system against standard tissue cultures obtained during the same surgical procedure. All patients had long bone fractures of the lower limbs and were assigned to the <jats:italic>FRI<\/jats:italic> or <jats:italic>Control group<\/jats:italic> based on the FRI consensus definition. The <jats:italic>FRI group<\/jats:italic> consisted of 24 patients with confirmed FRI and the <jats:italic>Control group<\/jats:italic> consisted of 21 patients with aseptic nonunion or chronic pain (in the absence of other suggestive\/confirmatory criteria). Standard tissue cultures and cultures harvested by the RIA\u2010system showed similar results. In the <jats:italic>FRI group<\/jats:italic>, standard tissue cultures and RIA cultures revealed relevant pathogens in 67% and 71% of patients, respectively. Furthermore, in four FRI patients, cultures obtained by the RIA\u2010system revealed <jats:italic>additional<\/jats:italic> relevant pathogens that were not found by standard tissue culturing, which contributed to the optimization of the treatment plan. In the <jats:italic>Control group<\/jats:italic>, there were no false\u2010positive RIA culture results. As a proof\u2010of\u2010concept, this cohort study showed that the RIA\u2010system could have a role in the diagnosis of FRI as an adjunct to standard tissue cultures. Since scientific evidence on the added value of the RIA\u2010system in the management of FRI is currently limited, further research on this topic is required before its routine application in clinical practice.<\/jats:p>","DOI":"10.1002\/jor.24816","type":"journal-article","created":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T17:51:57Z","timestamp":1596217917000},"page":"281-290","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Intramedullary tissue cultures from the Reamer\u2010Irrigator\u2010Aspirator system for diagnosing fracture\u2010related infection"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5096-5789","authenticated-orcid":false,"given":"Jolien","family":"Onsea","sequence":"first","affiliation":[{"name":"Department of Trauma Surgery University Hospitals Leuven Leuven Belgium"},{"name":"Department of Development and Regeneration, KU Leuven University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Pallay","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery University Hospitals Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Depypere","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University Hospitals Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2307-0397","authenticated-orcid":false,"given":"Thomas Fintan","family":"Moriarty","sequence":"additional","affiliation":[{"name":"AO Research Institute Davos Davos Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2597-7948","authenticated-orcid":false,"given":"Esther M.M.","family":"Van Lieshout","sequence":"additional","affiliation":[{"name":"Trauma Research Unit Department of Surgery, Erasmus MC University Medical Centre Rotterdam Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8942-1842","authenticated-orcid":false,"given":"William T.","family":"Obremskey","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery and Rehabilitation Vanderbilt University Medical Center Nashville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6295-9127","authenticated-orcid":false,"given":"An","family":"Sermon","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery University Hospitals Leuven Leuven Belgium"},{"name":"Department of Development and Regeneration, KU Leuven University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8061-1060","authenticated-orcid":false,"given":"Harm","family":"Hoekstra","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery University Hospitals Leuven Leuven Belgium"},{"name":"Department of Development and Regeneration, KU Leuven University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8448-5903","authenticated-orcid":false,"given":"Michael H.J.","family":"Verhofstad","sequence":"additional","affiliation":[{"name":"Trauma Research Unit Department of Surgery, Erasmus MC University Medical Centre Rotterdam Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1119-4918","authenticated-orcid":false,"given":"Stefaan","family":"Nijs","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery University Hospitals Leuven Leuven Belgium"},{"name":"Department of Development and Regeneration, KU Leuven University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4114-9093","authenticated-orcid":false,"given":"Willem\u2010Jan","family":"Metsemakers","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery University Hospitals Leuven Leuven Belgium"},{"name":"Department of Development and Regeneration, KU Leuven University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00402-019-03287-4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2016.09.019"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOT.0000000000001614"},{"key":"e_1_2_8_5_1","first-page":"257","article-title":"Intramedullary reaming in the treatment of chronic osteomyelitis of the femur and tibia","volume":"85","author":"Gualdrini G","year":"2000","journal-title":"Chir Organi Mov"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00636173"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOT.0b013e318038d952"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-200202000-00031"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-010-1189-z"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2017.04.025"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/BLO.0b013e318098673f"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2017.08.040"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2009.07.070"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00068-017-0821-7"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2019.05.014"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00068-016-0700-7"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13037-019-0215-3"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.2214\/AJR.18.20125"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejrad.2019.01.032"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00256-019-3152-4"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3055"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.CC.17.00154"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00013-11"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.04001.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0196051"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2015.03.019"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.08.006"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.7150\/jbji.22566"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.96B6.32244"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2015.07.027"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOT.0000000000000043"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.24170"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.100B7.BJJ-2018-0052.R1"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjor.24816","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.24816","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jor.24816","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.24816","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T13:14:22Z","timestamp":1693746862000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.24816"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,10]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/jor.24816"],"URL":"https:\/\/doi.org\/10.1002\/jor.24816","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,10]]},"assertion":[{"value":"2020-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}