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Eighty Lewis rats underwent ACL transection and were divided into four groups: (1) intra\u2010articular injection with phosphate\u2010buffered saline (PBS; <jats:italic>n<\/jats:italic>\u2009=\u200920), (2) intra\u2010articular injection of ECM\u2010B using aseptically processed ECM (ASEPTIC; <jats:italic>n<\/jats:italic>\u2009=\u200920), (3) intra\u2010articular injection of the ECM\u2010busing ECM powder sterilized with 15\u2009kGy electron beam irradiation (EBEAM; <jats:italic>n<\/jats:italic>\u2009=\u200920), and (4) intra\u2010articular injection of the ECM\u2010B using ECM powder sterilized with ethylene oxide (EO; <jats:italic>n<\/jats:italic>\u2009=\u200920). Twenty additional animals received capsulotomy only (SHAM). The animals were followed for 6 weeks and evaluations of gait, radiographs, and joint cartilage histology were performed. At 6 weeks, when compared to the SHAM group, the group treated with PBS had significantly worse gait and histologic changes, while the ASEPTIC group was not different from SHAM for either of these outcomes. When compared to the SHAM group, the EO group had similar gait outcomes, but greater histologic damage, and the EBEAM group had significantly worse gait and histological outcomes. The ECM\u2010B composite produced using aseptically processed ECM powder mitigated the gait and histologic changes associated with osteoarthritis after ACL transection in the rat; however, care must be taken when selecting a terminal sterilization method as this may affect the effectiveness of treatment.<\/jats:p>","DOI":"10.1002\/jor.25056","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T10:11:04Z","timestamp":1619691064000},"page":"573-583","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Terminal sterilization influences the efficacy of an extracellular matrix\u2010blood composite for treating posttraumatic osteoarthritis in the rat model"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9142-8496","authenticated-orcid":false,"given":"Naga Padmini","family":"Karamchedu","sequence":"first","affiliation":[{"name":"Department of Orthopaedics, Warren Alpert Medical School of Brown University Rhode Island Hospital  Providence Rhode Island USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7841-425X","authenticated-orcid":false,"given":"Braden C.","family":"Fleming","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Warren Alpert Medical School of Brown University Rhode Island Hospital  Providence Rhode Island USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5834-7934","authenticated-orcid":false,"given":"Benedikt L.","family":"Proffen","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Harvard Medical School Boston Children's Hospital  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J.","family":"Sant","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Harvard Medical School Boston Children's Hospital  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Portilla","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Harvard Medical School Boston Children's Hospital  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren R.","family":"Parola","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Warren Alpert Medical School of Brown University Rhode Island Hospital  Providence Rhode Island USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janine","family":"Molino","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Warren Alpert Medical School of Brown University Rhode Island Hospital  Providence Rhode Island USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5086-9511","authenticated-orcid":false,"given":"Martha M.","family":"Murray","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Harvard Medical School Boston Children's Hospital  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23557"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23341"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104681"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4085\/1062-6050-51.5.08"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00167-006-0087-9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:ABME.0000017541.82498.37"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.E.00539"},{"key":"e_1_2_8_9_1","first-page":"285","article-title":"Pathogenetic mechanisms of posttraumatic osteoarthritis: opportunities for early intervention","volume":"4","author":"Kramer WC","year":"2011","journal-title":"Int J Clin Exp Med"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23644"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12130"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2005.01.005"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.24800"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.21359"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.120"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23117"},{"key":"e_1_2_8_17_1","unstructured":"Services USDoHaH Administration FaD Health CfDaR et al.\u00a02016. 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