{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T04:54:04Z","timestamp":1773118444598,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T00:00:00Z","timestamp":1629244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["T32:AR007281"],"award-info":[{"award-number":["T32:AR007281"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007273","name":"Weill Cornell Medical College","doi-asserted-by":"publisher","award":["CTSA ULITR002384"],"award-info":[{"award-number":["CTSA ULITR002384"]}],"id":[{"id":"10.13039\/100007273","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Formalin\u2010fixed and paraffin\u2010embedded (FFPE) biospecimens are a valuable and widely\u2010available resource for diagnostic and research applications. With biobanks of tissue samples available in many institutions, FFPE tissues could prove to be a valuable resource for translational orthopaedic research. The purpose of this study was to characterize the molecular profiles and degree of histologic degeneration on archival fragments of FFPE human menisci obtained during arthroscopic partial meniscectomy. We used FFPE menisci for multiplexed gene expression analysis using the NanoString nCounter\u00ae platform, and for histological assessment using a quantitative scoring system. In total, 17 archival specimens were utilized for integrated histologic and molecular analyses. The median patient age was 22\u2009years (range: 14\u201362). We found that the genes with the highest normalized counts were those typically expressed in meniscal fibrocartilage. Gene expression differences were identified in patient cohorts based on age (\u226440\u2009years), including genes associated with the extracellular matrix and tissue repair. The majority of samples showed mild to moderate histologic degeneration. Based on these data, we conclude that FFPE human menisci can be effectively utilized for molecular evaluation following a storage time as long as 11 years.\u00a0<jats:bold>Statement of Clinical Significance:<\/jats:bold> The integration of histological and transcriptomic analyses described in this study will be useful for future studies investigating the basis for biological classification of meniscus specimens in patients. Further exploration into the genes and pathways uncovered by this study may suggest targets for biomarker discovery and identify patients at greater risk for osteoarthritis once the meniscus is torn.<\/jats:p>","DOI":"10.1002\/jor.25153","type":"journal-article","created":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T19:36:08Z","timestamp":1628537768000},"page":"1104-1112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Targeted transcriptomic analyses of RNA isolated from formalin\u2010fixed and paraffin\u2010embedded human menisci"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7395-226X","authenticated-orcid":false,"given":"Farrah A.","family":"Monibi","sequence":"first","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program Hospital for Special Surgery  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tania","family":"Pannellini","sequence":"additional","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program Hospital for Special Surgery  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1427-876X","authenticated-orcid":false,"given":"Brett","family":"Croen","sequence":"additional","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program Hospital for Special Surgery  New York New York USA"},{"name":"Drexel University College of Medicine  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4945-2994","authenticated-orcid":false,"given":"Miguel","family":"Otero","sequence":"additional","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program Hospital for Special Surgery  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Russell","family":"Warren","sequence":"additional","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program Hospital for Special Surgery  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0745-9880","authenticated-orcid":false,"given":"Scott A.","family":"Rodeo","sequence":"additional","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program Hospital for Special Surgery  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.24045"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27766"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2018.00073"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30137"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008207.2016.1243670"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0000000000000566"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12570"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.03.065"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-2378"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9904-0_10"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-011-1184-9"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-11-46"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.25047"},{"key":"e_1_2_9_15_1","article-title":"Knee fibrosis is associated with the development of osteoarthritis in a murine model of tibial compression","author":"Wang M","year":"2020","journal-title":"J Orthop Res"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.24434"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546520934766"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2011.05.008"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546506293700"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.37984"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-019-0062-y"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00402-011-1332-3"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008209809021499"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-199003000-00003"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2019.10.001"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.knee.2017.08.001"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1376333"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1394164"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1053\/joca.2000.0350"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1053\/joca.2000.0351"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.43.4.635"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780260111"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22787"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2013.11.002"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2018.59.6.760"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11845-008-0219-y"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1053\/joca.2002.0850"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.25980"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3515"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.86B7.14349"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1177\/03635465990270051601"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1177\/0095399703258743"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-199003000-00005"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2016.08.004"},{"key":"e_1_2_9_45_1","first-page":"205","article-title":"Sarcoma subgrouping by detection of fusion transcripts using NanoString nCounter technology. Pediatr Dev","volume":"22","author":"Sheth J","year":"2019","journal-title":"Pathology"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2017.09.007"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.70.7901"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12555"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2013.11.007"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI38248"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2018-214539"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.25153","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jor.25153","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.25153","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T23:33:27Z","timestamp":1693006407000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.25153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,18]]},"references-count":50,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1002\/jor.25153"],"URL":"https:\/\/doi.org\/10.1002\/jor.25153","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,18]]},"assertion":[{"value":"2021-03-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}