{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:35:51Z","timestamp":1790775351468,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T00:00:00Z","timestamp":1644278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Meteorin\u2010like protein (Metrnl), homologous to the initially identified neurotrophic factor Meteorin, is a secreted, multifunctional protein. Here we used mouse models to investigate Metrnl's role in skeletal development and bone fracture healing. During development\n                    <jats:italic>Metrnl<\/jats:italic>\n                    was expressed in the perichondrium and primary ossification center. In neonates, single cell RNA\u2010seq of diaphyseal bone demonstrated strongest expression of\n                    <jats:italic>Metrnl<\/jats:italic>\n                    transcript by osteoblasts. In vitro, Metrnl was osteoinductive, increasing osteoblast differentiation and mineralization in tissue culture models. In vivo, loss of Metrnl expression resulted in no change in skeletal metrics in utero, at birth, or during postnatal growth. Six\u2010week\u2010old Metrnl\u2010null mice displayed similar body length, body weight, tibial length, femoral length, BV\/TV, trabecular number, trabecular thickness, and cortical thickness as littermate controls. In 4\u2010month\u2010old mice, lack of Metrnl expression did not change structural stiffness, ultimate force, or energy to fracture of femora under 3\u2010point\u2010bending. Last, we investigated the role of Metrnl in bone fracture healing. Metrnl expression increased in response to tibial injury, however, loss of Metrnl expression did not affect the amount of bone deposited within the healing tissue nor did it change the structural parameters of healing tissue. This work identifies Metrnl as a dispensable molecule for skeletal development. However, the osteoinductive capabilities of Metrnl may be utilized to modulate osteoblast differentiation in cell\u2010based orthopedic therapies.\n                  <\/jats:p>","DOI":"10.1002\/jor.25286","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T05:33:20Z","timestamp":1643088800000},"page":"2510-2521","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["The role of Meteorin\u2010like in skeletal development and bone fracture healing"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3748-3627","authenticated-orcid":false,"given":"Rong","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Medicine Duke Molecular Physiology Institute  Durham North Carolina USA"},{"name":"Department of Orthopaedic Surgery Duke University  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhinav R.","family":"Balu","sequence":"additional","affiliation":[{"name":"Department of Medicine Duke Molecular Physiology Institute  Durham North Carolina USA"},{"name":"Department of Orthopaedic Surgery Duke University  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin H.","family":"Molitoris","sequence":"additional","affiliation":[{"name":"Department of Medicine Duke Molecular Physiology Institute  Durham North Carolina USA"},{"name":"Department of Orthopaedic Surgery Duke University  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"White","sequence":"additional","affiliation":[{"name":"Department of Medicine Duke Molecular Physiology Institute  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander G.","family":"Robling","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6625-2743","authenticated-orcid":false,"given":"Ugur M.","family":"Ayturk","sequence":"additional","affiliation":[{"name":"Department of Research Hospital for Special Surgery  New York City New York USA"},{"name":"Department of Orthopaedic Surgery Weill Cornell Medicine  New York City New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6827-1785","authenticated-orcid":false,"given":"Gurpreet S.","family":"Baht","sequence":"additional","affiliation":[{"name":"Department of Medicine Duke Molecular Physiology Institute  Durham North Carolina USA"},{"name":"Department of Orthopaedic Surgery Duke University  Durham North Carolina USA"},{"name":"Department of Pathology Duke University  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07666-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0164446"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600202"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2337\/db15-0274"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.065"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15301"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800435"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-020-0184-y"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.585644"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2015.02.010"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-014-0209-0"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2014.34"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-015-0032-3"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2388"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M601000200"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202001145R"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.129144"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2422"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1946"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr427"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.4052"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008200390152061"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2005.11.017"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910408300902"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2005.00389.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411000110030101"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411990100010401"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20091864"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2008.06.004"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117402"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e16-10-0716"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071294"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.14.8156"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2018.12.001"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.050815"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3683"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8131"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13112"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.orthres.2005.04.003.1100230610"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201906022"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI80313"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2017.02.012"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1997.12.1.24"},{"key":"e_1_2_8_46_1","first-page":"423","article-title":"The economic impact of geriatric hip fractures","volume":"28","author":"Youm T","year":"1999","journal-title":"Am J Orthop (Belle Mead NJ)"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.38643.663843.55"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.080606"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glp019"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-4356(03)00129-X"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.25286","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jor.25286","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.25286","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T12:19:45Z","timestamp":1692533985000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.25286"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,8]]},"references-count":49,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1002\/jor.25286"],"URL":"https:\/\/doi.org\/10.1002\/jor.25286","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,8]]},"assertion":[{"value":"2021-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}