{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T14:19:11Z","timestamp":1791641951048,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T00:00:00Z","timestamp":1669680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01 AR059088"],"award-info":[{"award-number":["R01 AR059088"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study aims to develop a comprehensive and easily executable histopathologic grading scheme for murine knee osteoarthritis (OA) using specific scoring criteria for both cartilage and periarticular changes, which may overcome important limitations of the existing grading systems. The new grading scheme was developed based on mouse knee OA models with observation periods up to 24 months of age (spontaneous OA) or 24\u2010week post\u2010injury (posttraumatic OA). Semi\u2010quantitative assessments of the histopathologic OA changes were applied to all four quadrants per femorotibial joint for 50 joints (200 quadrants) using specific scoring criteria rather than mild to severe grades. Scoring elements per quadrant were as follows: cartilage lesion (0\u20137), osteophyte (0\u20133), subchondral bone change (0\u20133), synovitis (0\u20133), and ectopic periarticular soft\u2010tissue chondrogenesis and ossification (0\u20133). The new histopathologic grading scheme had high intra\u2010 and interobserver reproducibility (correlation coefficients\n                    <jats:italic>r<\/jats:italic>\n                    \u2009&gt;\u20090.95) across experienced and novice observers. Sensitivity and reliability analyses confirmed the ability of the new scheme to detect minimal but significant OA progression (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) within a 2\u2010week interval and to accurately identify tissue\u2010 and quadrant\u2010specific OA severity within the joints. In conclusion, this study presents the first whole\u2010joint histopathologic grading scheme for murine knee OA that covers all\u2010stage osteoarthritic changes in all major joint tissues, including periarticular soft\u2010tissue ossification that is not included in any of the existing OA grading systems. This reproducible scheme is easy to execute and sensitive to minimal OA progression without using computer software, suitable for quick OA severity assessments of the entire femorotibial joint.\n                  <\/jats:p>","DOI":"10.1002\/jor.25482","type":"journal-article","created":{"date-parts":[[2022,11,12]],"date-time":"2022-11-12T10:13:59Z","timestamp":1668248039000},"page":"1407-1418","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A whole\u2010joint histopathologic grading system for murine knee osteoarthritis"],"prefix":"10.1002","volume":"41","author":[{"given":"Caleb W.","family":"Grote","sequence":"first","affiliation":[{"name":"Harrington Laboratory for Molecular Orthopedics, Department of Orthopedic Surgery University of Kansas Medical Center Kansas City Kansas USA"},{"name":"Department of Orthopedics and Musculoskeletal Sciences Children's Mercy Hospitals &amp; Clinics Kansas City Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Mackay","sequence":"additional","affiliation":[{"name":"Harrington Laboratory for Molecular Orthopedics, Department of Orthopedic Surgery University of Kansas Medical Center Kansas City Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Lu","sequence":"additional","affiliation":[{"name":"Harrington Laboratory for Molecular Orthopedics, Department of Orthopedic Surgery University of Kansas Medical Center Kansas City Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangliang","family":"Liu","sequence":"additional","affiliation":[{"name":"Harrington Laboratory for Molecular Orthopedics, Department of Orthopedic Surgery University of Kansas Medical Center Kansas City Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders R.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Kansas Medical Center Kansas City Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8626-5637","authenticated-orcid":false,"given":"Jinxi","family":"Wang","sequence":"additional","affiliation":[{"name":"Harrington Laboratory for Molecular Orthopedics, Department of Orthopedic Surgery University of Kansas Medical Center Kansas City Kansas USA"},{"name":"Department of Biochemistry &amp; Molecular Biology University of Kansas Medical Center Kansas City Kansas 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