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The need for surgical intervention is due, in part, to the inflammatory and catabolic environment that inhibits meniscus healing after injury. In other organ systems, healing is dependent on the migration of cells to the site of injury; however, in the meniscus, it is currently unknown how the microenvironment dictates cell migration in the postinjury inflamed setting. Here, we investigated how inflammatory cytokines alter meniscal fibrochondrocyte (MFC) migration and sensation of microenvironmental stiffness. We further tested whether an FDA approved interleukin\u20101 receptor antagonist (IL\u20101Ra; Anakinra) could rescue migratory deficits caused by inflammatory challenge. When cultured in the presence of inflammatory cytokines (tumor necrosis factor\u2010\u03b1 [TNF\u2010\u03b1] or interleukin\u20101\u03b2 [IL\u20101\u03b2]) for 1 day, MFC migration was inhibited for 3 days before returning to control levels at Day 7. This migratory deficit was clear in three\u2010dimensional as well, where fewer MFCs exposed to inflammatory cytokines migrated from a living meniscal explant compared with control. Notably, addition of IL\u20101Ra to MFCs previously exposed to IL\u20101\u03b2 restored migration to baseline levels. This study demonstrates that joint inflammation can have negative impacts on meniscus cell migration and mechanosensation, affecting their potential for repair, and that resolution of this inflammation with concurrent anti\u2010inflammatories can reverse these deficits. Future work will apply these findings to mitigate the negative consequences of joint inflammation and promote repair in a clinically relevant meniscus injury model.<\/jats:p>","DOI":"10.1002\/jor.25545","type":"journal-article","created":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T11:44:32Z","timestamp":1677843872000},"page":"2055-2064","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transient inhibition of meniscus cell migration following acute inflammatory challenge"],"prefix":"10.1002","volume":"41","author":[{"given":"Elisabeth A.","family":"Lemmon","sequence":"first","affiliation":[{"name":"University of Pennsylvania Perelman School of Medicine, Orthopaedic Surgery  Philadelphia Pennsylvania USA"},{"name":"Department of Veterans Affairs CMCVAMC  Philadelphia Pennsylvania USA"},{"name":"Department of Bioengineering University of Pennsylvania  Philadelphia Pennsylvania 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9537-603X","authenticated-orcid":false,"given":"Robert L.","family":"Mauck","sequence":"additional","affiliation":[{"name":"University of Pennsylvania Perelman School of Medicine, Orthopaedic Surgery  Philadelphia Pennsylvania USA"},{"name":"Department of Veterans Affairs CMCVAMC  Philadelphia Pennsylvania USA"},{"name":"Department of Bioengineering University of Pennsylvania  Philadelphia Pennsylvania 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