{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T20:15:17Z","timestamp":1762460117422,"version":"3.37.3"},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,3,26]],"date-time":"2023-03-26T00:00:00Z","timestamp":1679788800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,3,26]],"date-time":"2023-03-26T00:00:00Z","timestamp":1679788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W81XWH\u201017\u20101\u20100626"],"award-info":[{"award-number":["W81XWH\u201017\u20101\u20100626"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Composite tissue injuries (CTIs) in extremities include segmental bone defects (SBDs) and volumetric muscle loss. The objective of this study was to determine if skeletal muscle autografting with minced muscle grafts (MMGs) could improve healing in an SBD and improve muscle function in a porcine CTI model that includes an SBD and adjacent volumetric muscle loss injury. Adult Yucatan Minipigs were stratified into three groups including specimens with an isolated SBD, an SBD with volumetric muscle loss (CTI), and an SBD with volumetric muscle loss treated with MMG (CTI\u2009+\u2009MMG). Bone healing was quantified with serial x\u2010rays and postmortem computed tomography scanning. Muscle function was quantified with a custom in vivo force transducer. Muscle tissue content was determined by biochemical analyses and histology. Anterior cortex\u2010modified Radiographic Union Score for Tibia fractures (mRUSTs) decreased from 2.7 to 1.9 (<jats:italic>p<\/jats:italic>\u2009=\u20090.003) in CTI versus SBD animals. MMG improved anterior mRUST scores to 2.5 in CTI\u2009+\u2009MMG specimens (<jats:italic>p<\/jats:italic>\u2009=\u20090.030 compared to CTI specimens) and overall mRUST scores increased from 9.4 in CTI specimens to 11.1 in CTI\u2009+\u2009MMG specimens (<jats:italic>p<\/jats:italic>\u2009=\u20090.049). Residual strength deficits at euthanasia were 42% in SBD (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), 44% in CTI (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), and 48% in CTI\u2009+\u2009MMG (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) compared to preoperative values. There were no differences in strength deficits between the three groups. Biochemical and histologic analyses demonstrated scattered differences between the three groups compared to contralateral muscle. MMG improved bone healing. However, the primary cause of muscle dysfunction and biochemical changes was the presence of an SBD. Clinical significance: Early mitigation of SBDs may be necessary to prevent muscle damage and weakness in patients sustaining composite extremity trauma.<\/jats:p>","DOI":"10.1002\/jor.25551","type":"journal-article","created":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T07:17:05Z","timestamp":1678951025000},"page":"1890-1901","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Minced muscle autografting improves bone healing but not muscle function in a porcine composite injury model"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6354-7685","authenticated-orcid":false,"given":"Todd O.","family":"McKinley","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Indiana University School of Medicine IU Health Methodist Hospital Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4182-3244","authenticated-orcid":false,"given":"Roman N.","family":"Natoli","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Indiana University School of Medicine IU Health Methodist Hospital Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naveena B.","family":"Janakiram","sequence":"additional","affiliation":[{"name":"Department of Surgery Uniformed Services University of the Health Sciences and Walter Reed National Military Medical Center Bethesda Maryland USA"},{"name":"DoD\u2010VA Extremity Trauma &amp; Amputation Center of Excellence Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart J.","family":"Warden","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy, Indiana University School of Health and Human Sciences Indiana University Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robyn K.","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy, Indiana University School of Health and Human Sciences Indiana University Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachary","family":"Gunderson","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nichlaus","family":"Diggins","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seungyup","family":"Sun","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Kolettis","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Indiana University School of Medicine IU Health Methodist Hospital Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen M.","family":"Goldman","sequence":"additional","affiliation":[{"name":"Department of Surgery Uniformed Services University of the Health Sciences and Walter Reed National Military Medical Center Bethesda Maryland USA"},{"name":"DoD\u2010VA Extremity Trauma &amp; Amputation Center of Excellence Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3701-0950","authenticated-orcid":false,"given":"Christopher L.","family":"Dearth","sequence":"additional","affiliation":[{"name":"Department of Surgery Uniformed Services University of the Health Sciences and Walter Reed National Military Medical Center Bethesda Maryland USA"},{"name":"DoD\u2010VA Extremity Trauma &amp; Amputation Center of Excellence Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Mendenhall","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Indiana University School of Medicine Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caio","family":"Staut","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Indiana University School of Medicine IU Health Methodist Hospital Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7293-0088","authenticated-orcid":false,"given":"Melissa A.","family":"Kacena","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Indiana University School of Medicine IU Health Methodist Hospital Indianapolis Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin T.","family":"Corona","sequence":"additional","affiliation":[{"name":"Wake Forest University School of Medicine Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00936208"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.5435\/00124635-201102001-00002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOT.0b013e31821c0ec1"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1682\/JRRD.2014.07.0165"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2012.0290"},{"key":"e_1_2_9_7_1","first-page":"122","article-title":"Severe muscle trauma triggers heightened and prolonged local musculoskeletal inflammation and impairs adjacent tibia fracture healing","volume":"16","author":"Hurtgen BJ","year":"2016","journal-title":"J Musculoskelet Neuronal Interact"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/17453679909000960"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005131-200307000-00007"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016470216315"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e3182589e63"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0000000000001212"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.13362"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOT.0000000000000673"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40634-017-0112-6"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-13306-2"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/milmed\/usaa516"},{"key":"e_1_2_9_18_1","first-page":"2021","article-title":"Bone morphogenetic protein\u20102 rapidly heals two distinct critical sized segmental diaphyseal bone defects in a porcine model","volume":"24","author":"McKinley TO","year":"2021","journal-title":"Mil Med"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3944\/AOTT.2014.14.0026"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOT.0000000000000390"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3791\/62905"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/cts.12519"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-9120(96)00003-6"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.198.Supp.69.1"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20649"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-017-1617-y"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.25551","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.25551","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.25551","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T19:10:24Z","timestamp":1692213024000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.25551"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,26]]},"references-count":25,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1002\/jor.25551"],"URL":"https:\/\/doi.org\/10.1002\/jor.25551","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"type":"print","value":"0736-0266"},{"type":"electronic","value":"1554-527X"}],"subject":[],"published":{"date-parts":[[2023,3,26]]},"assertion":[{"value":"2022-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}