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In vivo, We established a standard OA model by performing the destabilized medial meniscus (DMM) surgery on C57BL\/6 mice, and assessed healing effect of Orientin by X\u2010ray imaging, histopathological analysis, immunohistochemical staining. Osteoarthritis (OA) is the most common form of degenerative joint disease in clinic and the chondrocyte inflammation plays the most important role in OA development. The natural flavonoid compound (Orientin) has anti\u2010inflammatory bioactive properties in the treatment of various diseases. But studies have not explored whether Orientin modulates OA progression. In this study, a significant suppression in IL\u20101\u03b2\u2010mediated pro\u2010inflammatory mediators and the degradation of cartilage extracellular matrix (ECM) was observed in vitro through qPCR, western blot analysis, ELISA, and immunofluorescent staining after the treatment with Orientin. In addition, Orientin abrogated DMM surgery induced cartilage degradation in mice, which was assessed by X\u2010ray imaging, histopathological analysis, immunohistochemical staining. Mechanistic studies showed that Orientin suppressed OA development by downregulating activation of NF\u2010\u03baB by activating Nrf2\/HO\u20101 axis and SIRT6 signaling pathway. These results provide evidence that Orientin serves as a potentially viable compound for the treatment of OA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jor.25573","type":"journal-article","created":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T02:51:26Z","timestamp":1682477486000},"page":"2405-2417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Orientin inhibits inflammation in chondrocytes and attenuates osteoarthritis through Nrf2\/NF\u2010\u03baB and SIRT6\/NF\u2010\u03baB pathway"],"prefix":"10.1002","volume":"41","author":[{"given":"Weiyi","family":"Xia","sequence":"first","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Wenzhou China"},{"name":"Key Laboratory of Orthopaedics of Zhejiang Province Wenzhou China"},{"name":"The Second School of Medicine Wenzhou Medical University Wenzhou 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