{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T15:19:38Z","timestamp":1748963978600,"version":"3.37.3"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K08AR073921"],"award-info":[{"award-number":["K08AR073921"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prosthetic joint infections (PJI) are associated with orthopaedic morbidity and mortality. Mitochondria, the \u201ccell's powerhouses,\u201d\u00a0are thought to play crucial roles in infection response and in increased risk of sepsis mortality. No current research discusses PJI's effect on mitochondrial function and a lack of understanding of immune\u2010infection interactions potentially hinders patient care. The purpose of this pilot study was to evaluate the impact of simulated PJI on local tissue mitochondrial function. Using an established prosthetic implant\u2010associated in vivo model, tissues were harvested from the surgical limb of a methicillin\u2010sensitive <jats:italic>Staphylococcus aureus<\/jats:italic> implant\u2010associated infection group (<jats:italic>n<\/jats:italic>\u2009=\u20096) and compared to a noninfected group (<jats:italic>n<\/jats:italic>\u2009=\u20096) at postoperative day (POD) 21. Using mitochondrial coupling assays, oxygen consumption rate and extracellular acidification rate were assessed in each group. Electron flow through mitochondrial complexes reflected group activity. Electron Paramagnetic Resonance (EPR) spectrometry measured the oxidizing potential of serum samples from infected versus noninfected groups. On POD21, colony\u2010forming units per gram of tissue showed 5\u2009\u00d7\u200910<jats:sup>9<\/jats:sup> in the infected group and 10<jats:sup>1<\/jats:sup> in the noninfected group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). Maximal respiration and oxygen consumption due to adenosine triphosphate synthesis were significantly lower in isolated mitochondria from infected limbs (<jats:italic>p<\/jats:italic>\u2009=\u20090.04). Both groups had similar complex I, III, IV, and V activity (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.1). Infected group EPR signal intensity reflecting reactive oxygen species levels was 1.31\u2009\u00b1\u20090.30 compared to 1.16\u2009\u00b1\u20090.28 (<jats:italic>p<\/jats:italic>\u2009=\u20090.73) in the noninfected group. This study highlights PJI's role in mammalian cell mitochondrial dysfunction and oxidative tissue damage, which can help develop interventions to combat PJI.<\/jats:p>","DOI":"10.1002\/jor.25696","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T06:14:13Z","timestamp":1696486453000},"page":"539-546","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A pilot study of mitochondrial response to an in vivo prosthetic joint <i>Staphylococcus aureus<\/i> infection model"],"prefix":"10.1002","volume":"42","author":[{"given":"Nour","family":"Bouji","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Health Sciences Center\u2014WVU School of Medicine Morgantown West Virginia 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