{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T01:12:13Z","timestamp":1768698733511,"version":"3.49.0"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T00:00:00Z","timestamp":1701043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR082167"],"award-info":[{"award-number":["R01AR082167"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to assess the viability of four Staphylococcal bacteriophages when exposed to different concentrations of commonly used lavage solutions in the surgical treatment of prosthetic joint infections (PJI). Four tailed Staphylococcal bacteriophages and six different lavage solutions (chlorhexidine 4%, hydrogen peroxide 3%, acetic acid 3%, povidone iodine 10%, sodium hypochlorite 0.5%, and Vashe solution) at 100%, 1%, and 0.01% concentrations were used in this experiment. In addition, the temporal impact of exposing bacteriophages to these lavage solutions was also evaluated at 5\u2010min exposures and 24\u2010h exposures. The results show that the titers of the four bacteriophages were statistically significantly decreased for all lavage solutions (100% and 1%) at 5\u2010min exposures and 24\u2010h exposures. However, with 0.01% concentrations of the lavage solutions, only acetic acid caused a statistically significant decrease in bacteriophage titers compared to normal saline control. Our findings suggest that tailed Staphylococcal bacteriophages do not remain stable in high concentrations of the most commonly used lavage solutions. However, at very dilute concentrations the bacteriophages do remain viable. This has important clinical ramifications in that it shows when using bacteriophage therapy for PJI it is critical to thoroughly wash out any lavage solutions before the introduction of therapeutic bacteriophages especially when acetic acid is used.<\/jats:p>","DOI":"10.1002\/jor.25731","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T13:25:24Z","timestamp":1700141124000},"page":"555-559","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The stability of Staphylococcal bacteriophages with commonly used prosthetic joint infection lavage solutions"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4977-0968","authenticated-orcid":false,"given":"James B.","family":"Doub","sequence":"first","affiliation":[{"name":"The Doub Translational Bacterial Research Laboratory University of Maryland School of Medicine Baltimore Maryland USA"},{"name":"Division of Clinical Care and Research, Institute of Human Virology University of Maryland School of Medicine Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessa","family":"Fogel","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery University of Maryland School of Medicine Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4511-1308","authenticated-orcid":false,"given":"Ken L.","family":"Urish","sequence":"additional","affiliation":[{"name":"Arthritis and Arthroplasty Design Group, The Bone and Joint Center Magee Women's Hospital of the University of Pittsburgh Medical Center Pittsburgh Pennsylvania USA"},{"name":"Department of Orthopaedic Surgery, Department of Bioengineering, Clinical and Translational Science Institute University of Pittsburgh Pittsburgh Pennsylvania 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