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Given that postmenopausal women are prone to suffer VD deficiency and intervertebral disc degeneration (IDD), this study is intended to investigate whether VD can delay IDD in ovariectomized rats by improving bone microstructure and antioxidant stress. Female Sprague\u2013Dawley rats were randomly allocated into four groups: sham, oophorectomy (OVX)+VD deficiency (VDD), OVX, and OVX+VD supplementation (VDS). In vivo, after a 6\u2010month intervention, imaging and pathology slice examinations showed that IDD induced by OVX was significantly alleviated in VDS and deteriorated by VDD. The expressions of aggrecan and Collagen II in intervertebral disc were reduced by OVX and VDD, and elevated by VDS. Compared with the OVX+VDD and OVX group vertebrae, OVX+VDS group vertebrae showed significantly improved endplate porosity and lumbar bone mineral density with increased percent bone volume and trabecular thickness. Furthermore, 1\u03b1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> restored the redox balance (total antioxidant capacity, ratio of oxidized glutathione\/glutathione) in the disc. The cocultivation of 1\u03b1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> and nucleus pulposus cells (NPCs) was conducted to observe its potential ability to resist excessive oxidative stress damage induced by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. In vitro experiments revealed that 1\u03b1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> reduced the senescence, apoptosis, and extracellular matrix degradation induced by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> in NPCs. In conclusion, VDS exhibits protective effects in OVX\u2010induced IDD, partly by regulating the redox balance and preserving the microstructure of endplate. This finding provides a new idea for the prevention and treatment of IDD.<\/jats:p>","DOI":"10.1002\/jor.25778","type":"journal-article","created":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T06:33:15Z","timestamp":1705386795000},"page":"1314-1325","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Vitamin D delays intervertebral disc degeneration and improves bone quality in ovariectomized rats"],"prefix":"10.1002","volume":"42","author":[{"given":"Hao\u2010Wei","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Spinal Surgery, Shanghai East Hospital, School of Medicine Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Yue","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery, Shanghai East Hospital, School of Medicine Tongji University Shanghai 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