{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T06:22:33Z","timestamp":1772518953360,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T00:00:00Z","timestamp":1712793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Linker of Nucleoskeleton and Cytoskeleton (LINC) complex is a crucial connective component between the nuclear envelope and the cytoskeleton involving various cellular processes including nuclear positioning, nuclear architecture, and mechanotransduction. How LINC complexes regulate bone formation in vivo, however, is not well understood. To start bridging this gap, here we created a LINC disruption murine model using transgenic mice expressing Cre recombinase enzyme under the control of the Osterix (Osx\u2010Cre) which is primarily active in pre\u2010osteoblasts and floxed Tg(CAG\u2010LacZ\/EGFP\u2010KASH2) mice. Tg(CAG\u2010LacZ\/EGFP\u2010KASH2) mice contain a lox\u2010STOP\u2010lox flanked LacZ gene which is deleted upon cre recombination allowing for the overexpression of an EGFP\u2010KASH2 fusion protein. This overexpressed protein disrupts endogenous Nesprin\u2010Sun binding leading to disruption of LINC complexes. Thus, crossing these two lines results in an\u00a0\n<jats:styled-content>O<\/jats:styled-content>sx\u2010\n<jats:styled-content>d<\/jats:styled-content>riven\u00a0\n<jats:styled-content>L<\/jats:styled-content>INC\u00a0\n<jats:styled-content>d<\/jats:styled-content>isruption (ODLD) specific to pre\u2010osteoblasts. In this study, we investigated how this LINC disruption affects exercise\u2010induced bone accrual. ODLD cells had decreased osteogenic and adipogenic potential in vitro compared to non\u2010disrupted controls and sedentary ODLD mice showed decreased bone quality at 8 weeks. Upon access to a voluntary running wheel,\u00a0ODLD animals showed increased running time and distance; however, our 6\u2010week exercise intervention did not significantly affect bone microarchitecture and bone mechanical properties.<\/jats:p>","DOI":"10.1002\/jor.25849","type":"journal-article","created":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T13:44:11Z","timestamp":1712843051000},"page":"2007-2016","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Osterix\u2010driven LINC complex disruption in vivo diminishes osteogenesis at 8 weeks\u00a0but not at 15 weeks"],"prefix":"10.1002","volume":"42","author":[{"given":"Scott","family":"Birks","sequence":"first","affiliation":[{"name":"Micron School of Materials Science and Engineering Boise State University Boise Idaho USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean","family":"Howard","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Biomedical Engineering Boise State University Boise Idaho USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"O'Rourke","sequence":"additional","affiliation":[{"name":"Biomedical Engineering The College of New Jersey Ewing Township New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William R.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department of Physical Therapy Indiana University Bloomington Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"Lau","sequence":"additional","affiliation":[{"name":"Biomedical Engineering The College of New Jersey Ewing Township New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1178-4942","authenticated-orcid":false,"given":"Gunes","family":"Uzer","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Biomedical Engineering Boise State University Boise Idaho USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-022-00190-4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13036-019-0197-9"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200509124"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02471"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.26.10.3738-3751.2006"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2021.116023"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2004"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep38063"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00622-5"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085161"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02480"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.22755"},{"key":"e_1_2_8_14_1","article-title":"Validation 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