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Here, we developed and validated a novel methodology for three\u2010dimensional structural analysis of meniscal vascular structures with high\u2010resolution microcomputed tomography (\u00b5CT). We collected porcine medial menisci from 10 neonatal (not\u2010developed meniscus, n\u2010) and 10 adults (fully developed meniscus, a\u2010). The menisci were cut into anatomical regions (anterior horn (n\u2010AH and\u00a0a\u2010AH), central body (n\u2010CB and\u00a0a\u2010CB), and posterior horn (n\u2010PH and\u00a0a\u2010PH). Specimens were cut in half, fixed, and one specimen underwent critical point drying and \u00b5CT imaging, while other specimen underwent immunohistochemistry and vascularity biomarker CD31 staining for validation of \u00b5CT. Parameters describing vascular structures were calculated from \u00b5CT. The vascular network in neonatal spread throughout meniscus, while in adult was limited to a few vessels in outer region, mostly on femoral side. n\u2010AH, n\u2010CB, and n\u2010PH had 20, 17, and 11 times greater vascular volume fraction than adult, respectively. Moreover, thickness of blood vessels, in three regions, was six times higher in adults than in neonatal. a\u2010PH appeared to have higher vascular fraction, longer and thicker blood vessels than both a\u2010AH and a\u2010CB. Overall, neonatal regions had a higher number of blood vessels, more branching, and higher tortuosity compared to adult regions. For the first time, critical point drying\u2010based \u00b5CT imaging allowed detailed three\u2010dimensional visualization and quantitative analysis of vascularized meniscal structures. We showed more vascularity in neonatal menisci, while adult menisci had fewer and thicker vascularity especially limited to the femoral surface.<\/jats:p>","DOI":"10.1002\/jor.25862","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T02:29:47Z","timestamp":1714444187000},"page":"2095-2105","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Age and anatomical region\u2010related differences in vascularization of the porcine meniscus using microcomputed tomography imaging"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8376-2454","authenticated-orcid":false,"given":"Ville\u2010Pauli","family":"Karjalainen","sequence":"first","affiliation":[{"name":"Research Unit of Health Sciences and Technology, Faculty of Medicine University of Oulu Oulu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina R.","family":"Herrera Millar","sequence":"additional","affiliation":[{"name":"Tissue Engineering and Biomaterials Lab IRCCS Ospedale Galeazzi Sant'Ambrogio Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Modina","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe M.","family":"Peretti","sequence":"additional","affiliation":[{"name":"Tissue Engineering and Biomaterials Lab IRCCS Ospedale Galeazzi Sant'Ambrogio Milan Italy"},{"name":"Department of Biomedical Sciences for Health University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margherita","family":"Pallaoro","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Sciences University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8345-4742","authenticated-orcid":false,"given":"Khaled","family":"Elkhouly","sequence":"additional","affiliation":[{"name":"Research Unit of Health Sciences and Technology, Faculty of Medicine University of Oulu Oulu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2850-5484","authenticated-orcid":false,"given":"Simo","family":"Saarakkala","sequence":"additional","affiliation":[{"name":"Research Unit of Health Sciences and Technology, Faculty of Medicine University of Oulu Oulu Finland"},{"name":"Department of Diagnostic Radiology Oulu University Hospital Oulu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6261-1286","authenticated-orcid":false,"given":"Ali","family":"Mobasheri","sequence":"additional","affiliation":[{"name":"Research Unit of Health Sciences and Technology, Faculty of Medicine University of Oulu Oulu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1674-3020","authenticated-orcid":false,"given":"Alessia","family":"Di Giancamillo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences for Health University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3348-5759","authenticated-orcid":false,"given":"Mikko A. 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