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A class of noncoding RNAs, microRNAs contribute to various diseases including musculoskeletal diseases. MicroRNA\u201026a (miR\u201026a) has been reported to be associated with fibrosis in several organs. This study aims to reveal the role of miR\u201026a on fibrosis in the shoulder capsule using a frozen shoulder model in miR\u201026a deficient (miR\u201026a KO) mice. MiR\u201026a KO and wild\u2010type (WT) mice were investigated using a frozen shoulder model. The range of motion (ROM) of the shoulder, histopathological changes such as synovitis, and fibrosis\u2010related gene expression in the model mice were evaluated to determine the role of miR\u201026a. In WT mice, both inflammatory cell infiltration and thickening of the inferior shoulder joint capsule were observed after 1 week of immobilization, and this thickening further progressed over the subsequent 6 weeks. However, the immobilized shoulder in miR\u201026a KO mice consistently exhibited significantly better ROM compared with WT mice at 1 and 6 weeks, and histological changes were significantly less severe. The expression of inflammation\u2010 and fibrosis\u2010related genes was decreased in the miR\u201026a KO mice compared with WT mice at 1 and 6 weeks. Together, miR\u201026a deficiency attenuated the severity of frozen shoulder in the immobilization model mouse. The present study suggests that miR\u201026a has the potential to be a target miRNA for therapeutic approach to frozen shoulder.<\/jats:p>","DOI":"10.1002\/jor.25940","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T14:45:09Z","timestamp":1721659509000},"page":"2623-2633","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["MicroRNA\u201026a deficiency attenuates the severity of frozen shoulder in a mouse immobilization model"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0695-6321","authenticated-orcid":false,"given":"Yasuhiko","family":"Sumimoto","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences Hiroshima University Hiroshima 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