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As opposed to standard\u2010of\u2010care systemic antibiotic prophylaxis for PJI, we developed and tested titanium femoral intramedullary implants with titania nanotubes (TNTs) coated with the antibiotic gentamicin and slow\u2010release agent chitosan through electrophoretic deposition (EPD) in a mouse model of PJI. We hypothesized that these implants would enable local gentamicin delivery to the implant surface and surgical site, effectively preventing bacterial colonization. In the mouse PJI model, C57BL\/6 mice received implants with TNTs coated with chitosan (chitosan group; control group) or with TNTs coated with chitosan and gentamicin (chitosan\u2009+\u2009gentamicin group; experimental group). Following implant placement, the surgical site was inoculated with 1\u2009\u00d7\u200910<jats:sup>3<\/jats:sup> CFUs of Xen36 bioluminescent <jats:italic>Staphylococcus aureus<\/jats:italic>. All the mice in the chitosan group and none in the chitosan\u2009+\u2009gentamicin group had evidence of infection based on CFU analysis and bioluminescence imaging through the 14\u2010day assessment postsurgery. Correspondingly, scanning electron microscopy analysis at the implant surface demonstrated bacterial biofilm only in the chitosan group. Furthermore, periosteal reaction and peri\u2010implant bone loss at the femur were significantly reduced in the chitosan\u2009+\u2009gentamicin group. The chitosan\u2009+\u2009gentamicin group had reduced pain behavior, improved weight\u2010bearing, and increased weight compared to the chitosan\u2010control group. This study provides preclinical evidence supporting the efficacy of implants with TNTs coated with chitosan and gentamicin through EPD for preventing bacterial colonization and biofilm formation in a mouse model of PJI.<\/jats:p>","DOI":"10.1002\/jor.26029","type":"journal-article","created":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T05:18:06Z","timestamp":1735708686000},"page":"671-681","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Electrophoretic Deposition of Gentamicin Into Titania Nanotubes Prevents Evidence of Infection in a Mouse Model of Periprosthetic Joint Infection"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0716-9816","authenticated-orcid":false,"given":"John L.","family":"Hamilton","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery Rush University Medical Center Chicago Illinois 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