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The anti\u2010inflammatory effect of liraglutide was assessed by measuring concentrations of inflammatory biomarker C\u2010C Motif Chemokine Ligand 2 (CCL2) in culture media of equine whole blood, peripheral blood mononuclear cells (PBMCs), chondrocytes, and synoviocytes, with or without lipopolysaccharide (LPS) or interleukin\u20101\u03b2. In the in vivo experiment, acute synovitis was bilaterally induced with 0.25\u2009ng LPS in the intercarpal joints of seven healthy Shetland ponies. The ponies were subsequently treated with either 6\u2009mg liraglutide or a placebo as a paired control in each joint. The impact of liraglutide on biomarkers associated with inflammation (including white blood cell count, total protein, CCL2, and bradykinin) and cartilage metabolism (such as glycosaminoglycans, general matrix metalloproteinase activity, carboxypropeptide type II collagen, and collagen\u2010cleavage neoepitope of type II collagen) was assessed across serial synovial fluid samples. Liraglutide was found to have an anti\u2010inflammatory effect by reducing CCL2 concentrations in culture media of whole blood, PBMCs, chondrocytes, and synoviocytes. In contrast, no significant differences in synovial fluid inflammatory nor cartilage metabolism biomarker levels were found between joints treated with LPS and 6\u2009mg liraglutide, versus LPS and placebo. In conclusion, liraglutide demonstrates the potential to attenuate inflammatory processes in joint cells. Additional research is necessary to validate its efficacy within the complex milieu of an inflamed joint.<\/jats:p>","DOI":"10.1002\/jor.26050","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T03:49:51Z","timestamp":1738727391000},"page":"893-903","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Anti\u2010Inflammatory Effects of Liraglutide in Equine Inflammatory Joint Models"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1552-6740","authenticated-orcid":false,"given":"Ann\u2010Sofie","family":"Scheike","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences Faculty of Veterinary Medicine, Utrecht University Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia","family":"Plomp","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Faculty of Veterinary Medicine, Utrecht University Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Carlotta","family":"Fugazzola","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Faculty of Veterinary Medicine, Utrecht University Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coralie","family":"Meurot","sequence":"additional","affiliation":[{"name":"4Moving Biotech Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Berenbaum","sequence":"additional","affiliation":[{"name":"4Moving Biotech Lille France"},{"name":"INSERM CRSA, AP\u2010HP Saint\u2010Antoine Hospital Sorbonne University Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul Ren\u00e9","family":"van Weeren","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Faculty of Veterinary Medicine, Utrecht University Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2333-7162","authenticated-orcid":false,"given":"Marianna Andriana","family":"Tryfonidou","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Faculty of Veterinary Medicine, Utrecht University Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes Hendrick","family":"von Hegedus","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Faculty of Veterinary Medicine, Utrecht University Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,4]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Global Burden of Disease Study 2019 (GBD 2019) Results. 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