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Greater hamstrings\/quadriceps co\u2010activation is common after ACLR and is, in part, responsible for impaired quadriceps function. Prolonged vibration of the hamstrings can alleviate reciprocal inhibition of the quadriceps and enhance quadriceps activation. We hypothesize that this will also reduce limb stiffness. Fourteen participants with unilateral ACLR, and 14 non\u2010injured individuals performed a single\u2010leg drop\u2010landing task, before and after 20\u2009min of hamstrings vibration. Limb stiffness, peak vertical ground reaction force, peak instantaneous loading rate, knee excursion, and peak knee extension moment were calculated during the loading phase of the drop\u2010landing task. The ACLR group had significantly greater limb stiffness (<jats:italic>p<\/jats:italic>\u2009=\u20090.002), peak vertical ground reaction force (<jats:italic>p<\/jats:italic>\u2009=\u20090.004), loading rate (<jats:italic>p<\/jats:italic>\u2009=\u20090.001), significantly less knee excursion (<jats:italic>p<\/jats:italic>\u2009=\u20090.009) and knee extension moment (<jats:italic>p<\/jats:italic>\u2009=\u20090.013) before vibration than non\u2010injured controls. Vibration significantly reduced limb stiffness (<jats:italic>p<\/jats:italic>\u2009=\u20090.001), peak vertical ground reaction force (<jats:italic>p<\/jats:italic>\u2009=\u20090.001), loading rate (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), significantly increased knee excursion (<jats:italic>p<\/jats:italic>\u2009=\u20090.01) and knee extension moment (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) in the ACLR group. No significant differences were found following vibration in the non\u2010injured control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical Significance<\/jats:title><jats:p>These results demonstrate that prolonged vibration of the hamstrings has the potential to mitigate the stiff limb loading strategy linked to knee osteoarthritis development, and may represent an effective adjunct therapy for ACLR rehabilitation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jor.26105","type":"journal-article","created":{"date-parts":[[2025,5,25]],"date-time":"2025-05-25T03:24:19Z","timestamp":1748143459000},"page":"1442-1453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Acute Prolonged Hamstrings Vibration Reduces Limb Stiffness Following Anterior Cruciate Ligament Reconstruction During a Single\u2010Limb Drop\u2010Jump 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