{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T04:58:39Z","timestamp":1776401919102,"version":"3.51.2"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Rotator cuff tendon injuries are common, particularly among the elderly. However, promising biomarkers to assess tendon degeneration and healing remain limited. This study aimed to identify dynamic biomarkers by analyzing temporal gene expression and histological changes in a chronic rotator cuff tear model. Using Sprague\u2013Dawley rats, a chronic injury was induced by detaching the supraspinatus tendon and inserting a silastic drain to inhibit spontaneous healing. Tendon samples were collected at multiple time points after injury and after repair for gene expression profiling and histological analysis. Human supraspinatus tendon samples (normal and degenerative) were acquired for validation. A three\u2010dimensional in vitro tendon construct model using human adipose\u2010derived stem cells was subjected to mechanical loading to investigate gene expression under controlled conditions. Tendon\u2010specific markers, including\n                    <jats:italic>Scleraxis<\/jats:italic>\n                    ,\n                    <jats:italic>Tenascin C<\/jats:italic>\n                    ,\n                    <jats:italic>and Collagen III<\/jats:italic>\n                    , were downregulated early after injury but demonstrated partial recovery with scar formation, limiting their utility as healing indicators. Conversely,\n                    <jats:italic>Collagen I<\/jats:italic>\n                    and\n                    <jats:italic>YAP1<\/jats:italic>\n                    showed significant downregulation during progressive tendon degeneration and marked upregulation following surgical repair. Histological improvements in collagen organization corresponded with\n                    <jats:italic>YAP1<\/jats:italic>\n                    re\u2010expression during the recovery phase.\n                    <jats:italic>YAP1<\/jats:italic>\n                    was consistently reduced in human degenerative tendons.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , mechanical loading enhanced the nuclear localization of YAP1 and upregulated tendon\u2010specific gene expression, confirming its mechanosensitivity. These findings establish YAP1 as a mechanotransducer and a promising biomarker of tendon degeneration and regeneration, with potential therapeutic relevance. This study demonstrated the translational value of YAP1 by integrating animal, human, and in vitro models to identify a robust marker of tendon remodeling.\n                  <\/jats:p>","DOI":"10.1002\/jor.70125","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T02:50:19Z","timestamp":1766976619000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Temporal Gene Expression Changes in Rotator Cuff Tendon Injury and Repair"],"prefix":"10.1002","volume":"44","author":[{"given":"Eui\u2010Sup","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Asan Medical Center University of Ulsan College of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6536-9536","authenticated-orcid":false,"given":"Jae Hee","family":"Choi","sequence":"additional","affiliation":[{"name":"Asan Institute for Life Sciences, Asan Medical Center University of Ulsan College of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Na","family":"Lee","sequence":"additional","affiliation":[{"name":"Asan Institute for Life Sciences, Asan Medical Center University of Ulsan College of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Se\u2010Ra","family":"Hwang","sequence":"additional","affiliation":[{"name":"Asan Institute for Life Sciences, Asan Medical Center University of Ulsan College of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1408-9048","authenticated-orcid":false,"given":"In Kyong","family":"Shim","sequence":"additional","affiliation":[{"name":"Asan Institute for Life Sciences, Asan Medical Center University of Ulsan College of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6181-9621","authenticated-orcid":false,"given":"Kyoung Hwan","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Asan Medical Center University of Ulsan College of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/2151458515583895"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.5397\/cise.2023.00430"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.5397\/cise.2022.01417"},{"key":"e_1_2_9_5_1","first-page":"1","article-title":"Rotator Cuff Tears in Elderly Patients: Is Repair Worthwhile?","volume":"6","author":"Nolte P.\u2010C.","year":"2020","journal-title":"Annals of Joint"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-55281-5"},{"key":"e_1_2_9_7_1","first-page":"103","article-title":"Current Concepts in Arthroscopic Rotator Cuff Repair","volume":"28","author":"Lee K.\u2010S.","year":"2025","journal-title":"Clinics in Shoulder and Elbow"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/23259671221084294"},{"key":"e_1_2_9_9_1","first-page":"196","article-title":"Prevalence and Characteristics of Asymptomatic Tears of the Rotator Cuff: An Ultrasonographic and Clinical Study","volume":"91","author":"Moosmayer S.","year":"2009","journal-title":"Journal of Bone and Joint Surgery. 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