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It has the potential to lead to post\u2010traumatic osteoarthritis. However, the effects of ACL injury on the bio\u2010composition and microstructure of the knee's collateral ligaments have been poorly explored. In this study, Fourier transform infrared (FTIR) imaging and quantitative polarized light microscopy (qPLM) were used to identify the respective changes in bio\u2010composition and collagen fiber arrangements of the knee's collateral ligaments. To mimic an ACL trauma, unilateral ACL transection surgery was performed on either the left or right knee of 6 mature New Zealand white rabbits. Lateral and medial collateral ligaments were harvested from the transected and contralateral knees 8 weeks after the ACL transection surgery. At the same time, collateral ligaments of 4 age\u2010matched, healthy rabbits were collected from the right and left knees. From acquired FTIR images, the relative collagen and proteoglycan contents of the collateral ligaments were estimated and compared between the transected, contralateral, and control knees. The results revealed lower collagen and higher proteoglycan content in ACL\u2010transected collateral ligaments compared to collateral ligaments of contralateral and control group knees. Additionally, qPLM revealed a more disorganized collagen fiber matrix, accompanied by increased crimp angles and longer crimp lengths following ACL transection. This study provides novel insight into the bio\u2010compositional and microstructural alterations of collateral ligaments following ACL injury, highlighting the importance of considering the structure\u2010function properties of collateral ligaments in treatment planning aimed at restoring normal knee joint function after ACL injury.<\/jats:p>","DOI":"10.1002\/jor.70132","type":"journal-article","created":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T04:59:13Z","timestamp":1767589153000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bio\u2010Compositional and Microstructural Changes in Rabbit Knee Collateral Ligaments Eight Weeks After Anterior Cruciate Ligament Transection"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7080-240X","authenticated-orcid":false,"given":"Anahita","family":"Gheisari","sequence":"first","affiliation":[{"name":"Research Unit of Health Sciences and Technology, Faculty of Medicine University of Oulu Oulu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8376-2454","authenticated-orcid":false,"given":"Ville\u2010Pauli","family":"Karjalainen","sequence":"additional","affiliation":[{"name":"Research Unit of Health Sciences and Technology, Faculty of Medicine University of Oulu Oulu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lassi","family":"Rieppo","sequence":"additional","affiliation":[{"name":"Polar Electro Oy Kempele Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sami","family":"Kauppinen","sequence":"additional","affiliation":[{"name":"Research Unit of Health Sciences and Technology, Faculty of Medicine University of Oulu Oulu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Sawatsky","sequence":"additional","affiliation":[{"name":"Faculty of Kinesiology, Human Performance Lab University of Calgary Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rami K.","family":"Korhonen","sequence":"additional","affiliation":[{"name":"Department of Applied Physics University of Eastern Finland Kuopio Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter","family":"Herzog","sequence":"additional","affiliation":[{"name":"Faculty of Kinesiology, Human Performance Lab University of Calgary Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2850-5484","authenticated-orcid":false,"given":"Simo","family":"Saarakkalaa","sequence":"additional","affiliation":[{"name":"Research Unit of Health Sciences and Technology, Faculty of Medicine University of Oulu Oulu Finland"},{"name":"Biocentre University of Oulu Oulu Finland"},{"name":"Department of Diagnostic Radiology Oulu University Hospital Oulu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikko A.J.","family":"Finnil\u00e4","sequence":"additional","affiliation":[{"name":"Research Unit of Health Sciences and Technology, Faculty of Medicine University of Oulu Oulu Finland"},{"name":"Biocentre University of Oulu Oulu Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuvashis","family":"Das Gupta","sequence":"additional","affiliation":[{"name":"Research Unit of Health Sciences and Technology, Faculty of Medicine University of Oulu Oulu Finland"},{"name":"Department of Biomedical Engineering Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ptsp.2012.05.001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/2325967118777823"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2003.008136"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546507307396"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2021.08.011"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546507301318"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546514530088"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546513517540"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjsports-2016-096031"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/23259671231214298"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jor.2022.07.024"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arthro.2024.06.016"},{"issue":"3","key":"e_1_2_10_14_1","first-page":"382","article-title":"The Effects of Transection of the Anterior Cruciate Ligament on Healing of the Medial Collateral Ligament","volume":"72","author":"Woo S. 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