{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T10:25:57Z","timestamp":1769682357995,"version":"3.49.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T00:00:00Z","timestamp":1768608000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR070613"],"award-info":[{"award-number":["R01AR070613"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["P30AR069655"],"award-info":[{"award-number":["P30AR069655"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Raman spectroscopy is a molecular fingerprinting technique that uses inelastic light scattering to characterize material composition and has been widely applied to bone quality assessment. In this ex vivo feasibility study, we tested whether Raman spectroscopy could predict femoral neck T\u2010scores (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200958) from specimens obtained during elective total hip arthroplasty and classify them as normal, osteopenic, or osteoporotic. Bone samples were excited using a near\u2010infrared, multiple spatial mode, semiconductor laser (830\u2009nm, 150\u2009mW), and scattered light was collected for spectral analysis. Custom MATLAB code calculated Raman outcomes. T\u2010scores were determined from bone mineral density acquired from dual\u2010energy X\u2010ray absorptiometry. Raman components were correlated with T\u2010score using simple linear or partial least squares regressions (PLSR). Bone health classifications were assessed through receiver operating characteristic curves (ROC), area under the ROC curves (AUC), and descriptive statistics. Simple linear regression models identified mineral maturity\/crystallinity (MMC) as the strongest single\u2010variable predictor of T\u2010score in males (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.20, 95% CI 0.00\u20130.43) and females (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.22, 95% CI 0.00\u20130.46). MMC showed fair discrimination between normal and osteopenic or osteoporotic groups in males (AUC\u2009=\u20090.76, 95% CI 0.56\u20130.97; 0.77, 95% CI 0.55\u20130.99) and females (AUC\u2009=\u20090.73, 95% CI 0.52\u20130.95; 0.70, 95% CI 0.47\u20130.92). PLSR (including mineral\u2010to\u2010matrix ratio, carbonate\u2010to\u2010phosphate ratio, MMC, and pyridinoline) slightly improved combined sex T\u2010score correlation (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.28, 95% CI 0.05\u20130.55) and male normal versus osteoporotic classification (AUC\u2009=\u20090.89, 95% CI 0.75\u20131.00). This ex vivo feasibility study demonstrates the potential of Raman spectroscopy to differentiate bone quality.\n                  <\/jats:p>","DOI":"10.1002\/jor.70145","type":"journal-article","created":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T06:08:51Z","timestamp":1768716531000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Raman Spectroscopy for Osteoporosis Assessment: Ex Vivo Feasibility of Molecular Fingerprinting of Bone"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3980-8966","authenticated-orcid":false,"given":"Anthony J.","family":"Yosick","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering University of Rochester Rochester New York USA"},{"name":"Center for Musculoskeletal Research, Department of Orthopaedics University of Rochester Medical Center Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8429-6281","authenticated-orcid":false,"given":"Andrew","family":"Rodenhouse","sequence":"additional","affiliation":[{"name":"Center for Musculoskeletal Research, Department of Orthopaedics University of Rochester Medical Center Rochester New York USA"},{"name":"Department of Orthopaedics University of Rochester Medical Center Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7141-5869","authenticated-orcid":false,"given":"Kyle","family":"Jerreld","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Rochester Rochester New York USA"},{"name":"Center for Musculoskeletal Research, Department of Orthopaedics University of Rochester Medical Center Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6638-9029","authenticated-orcid":false,"given":"Sashank","family":"Lekkala","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Rochester Rochester New York USA"},{"name":"Center for Musculoskeletal Research, Department of Orthopaedics University of Rochester Medical Center Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Massie","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Rochester Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"Berger","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Rochester Rochester New York USA"},{"name":"The Institute of Optics University of Rochester Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2197-2610","authenticated-orcid":false,"given":"Hani A.","family":"Awad","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Rochester Rochester New York USA"},{"name":"Center for Musculoskeletal Research, Department of Orthopaedics University of Rochester Medical Center Rochester New York USA"},{"name":"Department of Orthopaedics University of Rochester Medical Center Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constantinos","family":"Ketonis","sequence":"additional","affiliation":[{"name":"Center for Musculoskeletal Research, Department of Orthopaedics University of Rochester Medical Center Rochester New York USA"},{"name":"Department of Orthopaedics University of Rochester Medical Center Rochester New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,17]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"\u201cAmerican Academy of Orthopaedic Surgeons Osteoporosis\/Bone Health in Adults as a National Public Health Priority [Position Statement No. 1113] \u201d Rosemont IL American Academy of Orthopaedic Surgeons 2023 http:\/\/aaos.org\/globalassets\/about\/position-statements\/position-statement-1113-osteoporosis-bone-health-in-adults-as-a-national-public-health-priority_revised.v.3.pdf."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11657-023-01229-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(97)90022-X"},{"key":"e_1_2_8_5_1","first-page":"483","article-title":"Early Mortality After Hip Fracture: Is Delay Before Surgery Important?","volume":"87","author":"Moran C. G.","year":"2005","journal-title":"Journal of Bone & Joint Surgery"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2016.10.018"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2003.10.001"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2020.115490"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.9.004781"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.18.7.077001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2023.111852"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2021.110243"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgab180"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.02.011"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2696"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2024.124193"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-76337-2"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/35.9.813"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina58091207"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/1759720X14546350"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.70145","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jor.70145","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.70145","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:57:46Z","timestamp":1769648266000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.70145"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/jor.70145"],"URL":"https:\/\/doi.org\/10.1002\/jor.70145","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70145"}}