{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T15:07:06Z","timestamp":1779289626606,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR070750"],"award-info":[{"award-number":["R01AR070750"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["P30AR069619"],"award-info":[{"award-number":["P30AR069619"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The development of tendon hierarchical structure is dependent on collagen I assembly into fibrils and higher\u2010order assemblies, a process regulated by interactions involving collagen V, a quantitatively minor yet essential component of the tendon extracellular matrix. Collagen V critically regulates fibrillogenesis and is expressed throughout tendon development and maturation. Clinically, deficiency of collagen V manifests as classic Ehlers\u2010Danlos syndrome (cEDS), a disorder characterized by hyperextensible skin, joint hypermobility and instability, and impaired wound healing. Recent studies in mouse supraspinatus tendons\u2014which experience complex, region\u2010dependent loading environments at insertion and midsubstance\u2014demonstrate that developmental collagen V deficiency results in altered tendon structure and biomechanical function. However, differences in structure, mechanics, and gene expression resulting from reduced collagen V expression from embryonic development versus reduction during homeostasis have not been clearly delineated in mature tendons. To address this gap, this study elucidated the role of collagen V on region\u2010specific tendon structural, functional, and compositional properties in mature, day 150 supraspinatus tendons. Tendon\u2010targeted\n                    <jats:italic>Col5a1<\/jats:italic>\n                    deficiency from embryonic development resulted in substantial structural, mechanical, and transcriptional alterations, including disrupted fibril organization, compromised mechanical properties, and altered gene expression profiles. Conversely, acute deficiency and knockdown of\n                    <jats:italic>Col5a1<\/jats:italic>\n                    in mature tendons resulted in relatively minimal changes. Collectively, these findings identify a distinct and critical role for collagen V during tendon development, rather than homeostasis, in establishing region\u2010specific multiscale structural, mechanical, and molecular properties essential for mature supraspinatus tendon function.\n                  <\/jats:p>","DOI":"10.1002\/jor.70164","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:09:16Z","timestamp":1772986156000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Developmental, but not Homeostatic, Collagen V Expression Regulates Mature Murine Supraspinatus Tendon Structure, Function, and Gene Expression"],"prefix":"10.1002","volume":"44","author":[{"given":"Michael S.","family":"DiStefano","sequence":"first","affiliation":[{"name":"McKay Orthopedic Laboratory University of Pennsylvania Philadelphia Pennsylvania USA"},{"name":"Department of Bioengineering University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy D.","family":"Eekhoff","sequence":"additional","affiliation":[{"name":"McKay Orthopedic Laboratory University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie N.","family":"Weiss","sequence":"additional","affiliation":[{"name":"McKay Orthopedic Laboratory University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Courtney A.","family":"Nuss","sequence":"additional","affiliation":[{"name":"McKay Orthopedic Laboratory University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca L.","family":"Betts","sequence":"additional","affiliation":[{"name":"McKay Orthopedic Laboratory University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew F.","family":"Kuntz","sequence":"additional","affiliation":[{"name":"McKay Orthopedic Laboratory University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis J.","family":"Soslowsky","sequence":"additional","affiliation":[{"name":"McKay Orthopedic Laboratory University of Pennsylvania Philadelphia Pennsylvania USA"},{"name":"Department of Bioengineering University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22818"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2014.10.029"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2017.08.004"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.25299"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22893"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1129000"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008207.2024.2432324"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2015.01.031"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI12881"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.c.31548"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23246"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsfs.2015.0043"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.25740"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2022.111315"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0318809"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.70013"},{"key":"e_1_2_7_18_1","unstructured":"M.Favata2006. 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