{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T03:11:32Z","timestamp":1779333092733,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T00:00:00Z","timestamp":1776470400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Tendon injuries often result in prolonged healing and excessive scar tissue formation due to chronic hypoxia in this poorly perfused tissue. Hyperbaric oxygen therapy (HBOT), a non\u2010invasive treatment currently used for chronic wounds, may improve tendon healing by relieving hypoxia. However, the effects of HBOT on tendon cells, particularly tenocytes, remain poorly understood. This study investigated the in vitro effects of HBOT on murine Achilles tenocytes cultured under basal and inflammatory conditions induced by interleukin\u20101 beta (IL\u20101\u03b2). Tenocytes were exposed to 30, 60, or 90\u2009min of HBOT at 2.5 atmospheres absolute (ATA) and assessed for changes in in vitro wound healing using a scratch assay, mitochondrial activity, cell viability, collagen deposition, and gene expression at 24\u2010 and 72\u2010h post\u2010treatment. HBOT increased collagen deposition, while transiently suppressing gene expression of collagen types I and III immediately following HBOT treatment, along with reduced expression of the oxygen responsive gene HIF\u20101\u03b1. Mitochondrial activity increased significantly at 24\u2009h following longer HBOT exposure but remained unchanged with IL\u20101\u03b2. Cell viability remained high across all groups, although HBOT\u2010treated wells visually showed slightly more dead cells. Inflammatory conditions revealed a significant reduction in wound closure in HBOT\u2010treated tenocytes compared to controls. These findings suggest that HBOT can modulate mitochondrial activity, extracellular matrix (ECM) production, and gene expression in tenocytes, with differential effects in the presence of inflammation. Overall, this study provides new insights into the cellular effects of HBOT on tendon biology and supports further investigation into its therapeutic potential for tendon repair.<\/jats:p>","DOI":"10.1002\/jor.70204","type":"journal-article","created":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T05:09:36Z","timestamp":1776575376000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vitro Modulation of Murine Tenocyte Behavior by Hyperbaric Oxygen Therapy"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4772-663X","authenticated-orcid":false,"given":"Afton K.","family":"Limberg","sequence":"first","affiliation":[{"name":"Thayer School of Engineering at Dartmouth College Hanover New Hampshire USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lily M.","family":"Giurleo","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering at Dartmouth College Hanover New Hampshire USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering at Dartmouth College Hanover New Hampshire USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amritha","family":"Anup","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering at Dartmouth College Hanover New Hampshire USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jay C.","family":"Buckey","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering at Dartmouth College Hanover New Hampshire USA"},{"name":"Dartmouth Health Lebanon New Hampshire USA"},{"name":"Geisel School of Medicine at Dartmouth College Lebanon New Hampshire USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frances D.","family":"Faro","sequence":"additional","affiliation":[{"name":"Dartmouth Health Lebanon New Hampshire USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas W.","family":"Van Citters","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering at Dartmouth College Hanover New Hampshire USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3094-5537","authenticated-orcid":false,"given":"Katherine R.","family":"Hixon","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering at Dartmouth College Hanover New Hampshire USA"},{"name":"Geisel School of Medicine at Dartmouth College Lebanon New Hampshire USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1302\/2058-5241.2.160075"},{"issue":"6993","key":"e_1_2_7_3_1","first-page":"1534","article-title":"Assessing Health Needs in Primary Care","volume":"310","author":"Group BMJP","year":"1995","journal-title":"BMJ"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13010086"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-016-2461-3"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/BIO03904034"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.676782"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10060958"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010082"},{"key":"e_1_2_7_10_1","unstructured":"Commissioner of the FDA. Hyperbaric Oxygen Therapy: Get the Facts.2021."},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.25787"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20360"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-0266(01)00094-8"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3944\/AOTT.2012.2653"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-024-07694-6"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.4103\/mgr.MEDGASRES-D-24-00034"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.11070"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947603518764281"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21041452"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1302\/2046-3758.612.BJR-2017-0112.R1"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2005.08.006"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regen.2022.100059"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-39370-4"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.31168"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-91914-9"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pbj.0000000000000176"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.30"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.05.122"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.24538"},{"key":"e_1_2_7_30_1","unstructured":"Unlock Energy Production With Hyperbaric Oxygen Therapy.2023 https:\/\/hyperbaricoxygeninstitute.com\/hyperbaric-oxygen-therapy-increased-energy-production\/."},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25020777"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015832726054"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1524-475x.1999.00053.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2024.578367"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12253"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcws.2010.04.001"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.70204","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jor.70204","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.70204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T02:34:49Z","timestamp":1779330889000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.70204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":35,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/jor.70204"],"URL":"https:\/\/doi.org\/10.1002\/jor.70204","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2025-06-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70204"}}