{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T06:02:14Z","timestamp":1779775334150,"version":"3.53.1"},"reference-count":67,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T00:00:00Z","timestamp":1778371200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF21OC0065373"],"award-info":[{"award-number":["NNF21OC0065373"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Proteoglycan loss in articular cartilage is a hallmark of post\u2010traumatic osteoarthritis, and it can affect both chondrocyte biomechanics and tissue health. Previously, we found that 4 weeks after anterior cruciate ligament transection (ACLT) in rabbits, proteoglycan loss was less pronounced in the pericellular matrix (PCM) than in the extracellular matrix (ECM), resulting in a greater PCM\u2010to\u2010ECM proteoglycan content ratio compared to controls. Here, we investigated whether this pattern is already present 2 weeks post\u2010ACLT and whether changes in the PCM\u2010to\u2010ECM proteoglycan content ratio are associated with chondrocyte volumetric strain in superficial cartilage during tissue loading. Unilateral ACLT was performed in eight skeletally mature rabbits. Two weeks post\u2010surgery, cartilage from load\u2010bearing sites of the operated and contralateral knees was collected. Age\u2010matched, non\u2010operated knees from separate animals served as controls. Proteoglycan content in the chondrocyte microenvironment was analyzed in the superficial and middle zones via digital densitometry. Volumetric strain of superficial zone chondrocytes was also measured using two\u2010photon confocal microscopy during compressive loading. In the superficial zone, the PCM\u2010to\u2010ECM proteoglycan content ratio was greater in the ACLT knees than in controls at the lateral tibial plateau (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.043), but this was not clearly linked to changes in chondrocyte volumetric strain. In the middle zone, the PCM\u2010to\u2010ECM proteoglycan content ratio was greater in the ACLT knees than in controls for the medial femoral condyle, femoral groove, and patella (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). These results indicate preferential ECM proteoglycan loss relative to the PCM in early post\u2010traumatic osteoarthritis, especially in the middle zone.\n                  <\/jats:p>","DOI":"10.1002\/jor.70217","type":"journal-article","created":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T10:18:16Z","timestamp":1778408296000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Early Post\u2011Traumatic Osteoarthritis Features Greater Cartilage Extracellular Matrix Proteoglycan Loss Compared to the Pericellular Matrix"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-0709-2467","authenticated-orcid":false,"given":"Rasmus K.","family":"Hiltunen","sequence":"first","affiliation":[{"name":"Center of Oncology, Kuopio University Hospital Wellbeing Services County of North Savo Kuopio Finland"},{"name":"Department of Technical Physics University of Eastern Finland Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6124-1170","authenticated-orcid":false,"given":"Nina","family":"Pesonen","sequence":"additional","affiliation":[{"name":"Center of Oncology, Kuopio University Hospital Wellbeing Services County of North Savo Kuopio Finland"},{"name":"Department of Technical Physics University of Eastern Finland Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4386-0716","authenticated-orcid":false,"given":"Simo P.","family":"Ojanen","sequence":"additional","affiliation":[{"name":"Department of Technical Physics University of Eastern Finland Kuopio Finland"},{"name":"Department of Medical Physics Hospital Nova of Central Finland, Wellbeing Services County of Central Finland Jyv\u00e4skyl\u00e4 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3348-5759","authenticated-orcid":false,"given":"Mikko A. J.","family":"Finnil\u00e4","sequence":"additional","affiliation":[{"name":"Biocenter Oulu University of Oulu Oulu Finland"},{"name":"Research Unit of Health Sciences and Technology University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1526-5784","authenticated-orcid":false,"given":"Sanna","family":"Palosaari","sequence":"additional","affiliation":[{"name":"Research Unit of Translational Medicine, Faculty of Medicine University of Oulu Oulu Finland"},{"name":"Medical Research Center Oulu University Hospital and University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5341-0033","authenticated-orcid":false,"given":"Walter","family":"Herzog","sequence":"additional","affiliation":[{"name":"Human Performance Laboratory, Faculty of Kinesiology University of Calgary Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2850-5484","authenticated-orcid":false,"given":"Simo","family":"Saarakkala","sequence":"additional","affiliation":[{"name":"Biocenter Oulu University of Oulu Oulu Finland"},{"name":"Research Unit of Health Sciences and Technology University of Oulu Oulu Finland"},{"name":"Medical Research Center Oulu University Hospital and University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3486-7855","authenticated-orcid":false,"given":"Rami K.","family":"Korhonen","sequence":"additional","affiliation":[{"name":"Department of Technical Physics University of Eastern Finland Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9150-9253","authenticated-orcid":false,"given":"Juuso T. J.","family":"Honkanen","sequence":"additional","affiliation":[{"name":"Center of Oncology, Kuopio University Hospital Wellbeing Services County of North Savo Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9684-6902","authenticated-orcid":false,"given":"Petri","family":"Tanska","sequence":"additional","affiliation":[{"name":"Center of Oncology, Kuopio University Hospital Wellbeing Services County of North Savo Kuopio Finland"},{"name":"Department of Technical Physics University of Eastern Finland Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1088\/1361-6463\/aa6e18"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1941738109350438"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2013.03.009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.42089"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/rmdopen-2016-000279"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology9080194"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-021-00360-w"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0838.2009.00912.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380410"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.25009"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/0006355X2002039001002004"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0196203"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/jdb3040177"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2018.05.008"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.03.037"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb07700.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1346.011"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2007.01.009"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/164146"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2020.109648"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2007.09.002"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2018.11.024"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2020.05.005"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.08.001"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0049-0172(90)90081-P"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100150207"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2012.12.001"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2014.04.010"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1063-4584(05)80318-8"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2005.11.010"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2019.109450"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2005.02.001"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12944-019-1008-5"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medengphy.2009.05.013"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2016.06.033"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23650"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00501401"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02331378"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2014.08.009"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2013.09.021"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215549904701212"},{"issue":"6","key":"e_1_2_9_43_1","first-page":"511","article-title":"Perlecan: An Important Component of the Cartilage Pericellular Matrix","volume":"2","author":"Gomes R.","year":"2002","journal-title":"Journal of Musculoskeletal & Neuronal Interactions"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.103.4.1101"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2016.09.003"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(02)00034-3"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3390571"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100020301"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270405"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2017.02.122"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2017.02.004"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b04477"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2021.03.019"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2020.11.002"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.24036"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-008-0744-z"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-019-2566-4"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008208309015010"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10237-016-0817-y"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100130315"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10237-012-0409-4"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008207.2014.912645"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.09.002"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2009.08.003"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.39034"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2013.08.026"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2024.09.006"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2022.111336"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.70217","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jor.70217","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jor.70217","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T05:02:31Z","timestamp":1779771751000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jor.70217"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":67,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/jor.70217"],"URL":"https:\/\/doi.org\/10.1002\/jor.70217","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2025-10-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70217"}}