{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T18:40:58Z","timestamp":1792435258971,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24K12357"],"award-info":[{"award-number":["JP24K12357"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Meniscal calcification is increasingly recognized as a pathological feature associated with the progression of osteoarthritis (OA). However, the molecular mechanisms underlying this process remain poorly understood. This study aimed to investigate the involvement of Wnt\/\u03b2\u2010catenin signaling and its extracellular antagonist frizzled\u2010related protein B (FRZB) in pathological calcification of degenerative human menisci. Human meniscal specimens were obtained from patients with knee OA and non\u2010OA controls and analyzed histologically and immunohistochemically for calcification and expression of hypertrophic\/calcification\u2010associated markers, \u03b2\u2010catenin, and FRZB. An in vitro calcification model using primary human meniscal cells was established, and the role of FRZB was examined by supplementation with recombinant FRZB or shRNA\u2010mediated FRZB knockdown. Calcification was assessed with Alizarin Red S staining, and expression of Wnt\/\u03b2\u2010catenin signaling\u2010related and hypertrophic\/calcification\u2010associated genes was analyzed using quantitative PCR and western blotting. Degenerative menisci exhibited prominent calcium deposition accompanied by increased expressions of RUNX2, collagen type X, and MMP\u201013. Calcified menisci showed enhanced \u03b2\u2010catenin expression and reduced FRZB expression compared with non\u2010calcified menisci. In an in vitro model, meniscal cell calcification was accompanied by downregulation of FRZB and activation of Wnt\/\u03b2\u2010catenin signaling, along with upregulation of RUNX2, collagen type X, and MMP\u201013. Recombinant FRZB attenuated Wnt\/\u03b2\u2010catenin signaling activity and reduced calcification, whereas FRZB knockdown enhanced Wnt\/\u03b2\u2010catenin signaling and increased calcium deposition. Reduced FRZB expression was associated with enhanced Wnt\/\u03b2\u2010catenin signaling activity and increased calcification in degenerative menisci. The findings suggest a potential inhibitory role for FRZB in the context of Wnt\/\u03b2\u2010catenin signaling associated meniscal calcification.<\/jats:p>","DOI":"10.1002\/jor.70295","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:27:30Z","timestamp":1791433650000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reduced Frizzled\u2010Related Protein B Expression Is Associated With Wnt\/\u03b2\u2010Catenin Signaling Activation and Calcification in Degenerative Menisci"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8422-5053","authenticated-orcid":false,"given":"Yusuke","family":"Inoue","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery and Musculoskeletal Science, Graduate School of Medicine Yokohama City University Yokohama Kanagawa Japan"},{"name":"Department of Orthopaedic Surgery Yokohama City University 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