{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T16:52:14Z","timestamp":1781801534662,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,1,6]],"date-time":"2019-01-06T00:00:00Z","timestamp":1546732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471096"],"award-info":[{"award-number":["81471096"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aspenjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Parenter Enteral Nutr"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Evidence has provided support for the beneficial effects of milk fat globule membrane (MFGM) on inflammation in the intestinal tract. The objective of this study was to investigate the effects of MFGM on a rat model of necrotizing enterocolitis (NEC) and its potential mechanism of action.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty\u2010two newborn Sprague Dawley rats were randomly divided into 4 experimental groups: Breast\u2010fed normal, formula fed (FF), FF + 6 g\/L MFGM, and FF + 12 g\/L MFGM. The FF rats and the FF rats supplemented with MFGM were exposed to asphyxia\/cold stress to induce NEC. Body weight, histological score, survival time, oxidative stress injury, enterocyte proliferation\/apoptosis, and inflammatory response were assessed. Meanwhile, lipopolysaccharide (LPS)\u2010stimulated IEC\u20106 enterocytes were used as a model to test the anti\u2010inflammatory effects of MFGM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Supplementation with 12 g\/L MFGM alleviated body weight loss, reduced the incidence of NEC, increased the survival rate, and attenuated the severity of bowel damage in the NEC rat model. Furthermore, 12 g\/L MFGM administration inhibited the protein expression of toll\u2010like receptor 4 (TLR4) in the animal model. In IEC\u20106 enterocytes, the upregulation of TLR4, myeloid differentiation primary response gene 88 (MyD88), phosphorylated nuclear factor\u2010\u03baB (NF\u2010\u03baB) p65 subunit, and the nuclear translocation of NF\u2010\u03baBp65 induced by LPS was partially inhibited by MFGM pretreatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings suggested that MFGM has beneficial effects on neonatal rats with NEC by suppressing inflammation via the TLR4\/MyD88\/NF\u2010\u03baB pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jpen.1496","type":"journal-article","created":{"date-parts":[[2019,1,6]],"date-time":"2019-01-06T21:55:32Z","timestamp":1546811732000},"page":"863-873","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Milk Fat Globule Membrane Ameliorates Necrotizing Enterocolitis in Neonatal Rats and Suppresses Lipopolysaccharide\u2010Induced Inflammatory Response in IEC\u20106 Enterocytes"],"prefix":"10.1002","volume":"43","author":[{"given":"Dandan","family":"Zhang","sequence":"first","affiliation":[{"name":"Shanghai Institute for Pediatric Research Xinhua Hospital School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wen","sequence":"additional","affiliation":[{"name":"Shanghai Institute for Pediatric Research Xinhua Hospital School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiefei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shanghai Institute for Pediatric Research Xinhua Hospital School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Cai","sequence":"additional","affiliation":[{"name":"Shanghai Institute for Pediatric Research Xinhua Hospital School of Medicine 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