{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T16:51:25Z","timestamp":1781801485459,"version":"3.54.5"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T00:00:00Z","timestamp":1594944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aspenjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Parenter Enteral Nutr"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Clinical research reveals that colon plays an important role in mitigating the effects of short\u2010bowel syndrome (SBS). Previously, we showed that the milk fat globule membrane (MFGM) had protective effects on gut barrier integrity in the rat SBS model. Here, we used the same rat model to investigate the effects of enteral MFGM supplementation on gut microbiota and colonic\u2013mucus\u2010barrier function and its related mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We randomly divided 24 male Sprague\u2010Dawley rats into 3 groups: Sham, SBS (rats with massive small\u2010bowel resection), and SBS+MFGM (SBS rats supplemented with 1.5 g\/kg\/d MFGM). We then evaluated gut permeability, crypt depth, goblet\u2010cell count, mucin 1 (MUC1), mucin 2 (MUC2), microbiota, short\u2010chain fatty acids, and protein expressions of nucleotide\u2010binding oligomerization domain, leucine\u2010rich repeat, and pyrin domain\u2010containing protein 6 (NLRP6) pathway of the colon.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with SBS rats, SBS+MFGM rats exhibited lower intestinal permeability, increased crypt depth, more goblet cells, and more MUC1\/MUC2\u2010positive cells. The SBS+MFGM group also had greater Firmicutes abundance and lower acetate concentration (<jats:italic>P<\/jats:italic> &lt; .05). Sham rats had significantly lower Bacteroidetes abundance than SBS rats, but SBS+MFGM and SBS groups did not differ. Additionally, the SBS+MFGM group had higher NLRP6 and interleukin (IL)\u201018 expression but lower IL\u20101\u03b2 and Caspase\u20101 (cysteinyl aspartate\u2010specific protease\u20101) expression than the SBS group (<jats:italic>P<\/jats:italic> &lt; .05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Supplementation of MFGM modulates gut microbiota composition in SBS, possibly through strengthening the colonic mucus barrier and regulation of NLRP6 inflammasome.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jpen.1956","type":"journal-article","created":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T11:24:27Z","timestamp":1593689067000},"page":"916-925","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Milk Fat Globule Membrane Enhances Colonic\u2013Mucus\u2010Barrier Function in a Rat Model of Short\u2010Bowel Syndrome"],"prefix":"10.1002","volume":"45","author":[{"given":"Zhicai","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Clinical Nutrition Xin Hua Hospital affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition Xin Hua Hospital affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition Xin Hua Hospital affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingya","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition Xin Hua Hospital affiliated to Shanghai Jiao 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