{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:11:34Z","timestamp":1760872294705,"version":"build-2065373602"},"reference-count":50,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T00:00:00Z","timestamp":1627257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aspenjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Parenter Enteral Nutr"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Rapid loss of lean mass during catabolic states is associated with impaired convalescence and increased mortality rates. An understanding of metabolic pathways related to lean mass is needed to enable future interventions designed to combat malnutrition. This study assessed the plasma metabolome in relation to lean mass in clinically stable working adults in a US cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This cross\u2010sectional study included 180 adults (mean \u00b1 SD, aged 49.7 \u00b1 10.0 years; body mass index, 27.3 \u00b1 5.5 kg\/m<jats:sup>2<\/jats:sup>; 64% female [n=116]). Fasting plasma was analyzed using high\u2010resolution metabolomics (HRM) via liquid chromatography\/mass spectrometry. Lean mass was assessed by dual\u2010energy x\u2010ray absorptiometry and expressed as lean mass index (LMI, lean mass kg\/height m<jats:sup>2<\/jats:sup>). Multiple linear regression, metabolic pathway enrichment, and module analyses were used to characterize systemic metabolism associated with LMI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of 5360 metabolites used in analyses, 593 were related to LMI, either upregulated or downregulated (<jats:italic>P<\/jats:italic> &lt; .05). These were enriched within 11 metabolic pathways, including branched\u2010chain amino acid degradation, metabolism of alanine and aspartate and other amino acids, butyrate, purines, and niacin metabolism. Module analysis revealed central associations between LMI and L\u2010glutamate, L\u2010leucine\/L\u2010isoleucine, L\u2010valine, L\u2010phenylalanine, L\u2010methionine, and L\u2010aspartate, among other validated metabolites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These novel plasma HRM data demonstrate the wide\u2010reaching associations of lean mass with systemic metabolism in a single snapshot. Such data may inform targeted nutrition support interventions designed to mitigate loss of lean mass and promote regaining skeletal muscle mass and function after illness or injury.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jpen.2201","type":"journal-article","created":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T18:40:09Z","timestamp":1623350409000},"page":"1635-1644","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Plasma high\u2010resolution metabolomic phenotyping of lean mass in a United States adult cohort"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8998-4792","authenticated-orcid":false,"given":"Moriah P.","family":"Bellissimo","sequence":"first","affiliation":[{"name":"Division of Cardiology, Department of Internal Medicine, Pauley Heart Center Virginia Commonwealth University School of Medicine  Richmond Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dean P.","family":"Jones","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Emory Center for Clinical and Molecular Nutrition Emory University  Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg S.","family":"Martin","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Emory Center for Clinical and Molecular Nutrition Emory University  Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica A.","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Emory Center for Clinical and Molecular Nutrition Emory University  Atlanta Georgia USA"},{"name":"Division of Endocrinology, Metabolism, and Lipids, Department of Medicine Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas R.","family":"Ziegler","sequence":"additional","affiliation":[{"name":"Emory Center for Clinical and Molecular Nutrition Emory University  Atlanta Georgia USA"},{"name":"Division of Endocrinology, Metabolism, and Lipids, Department of Medicine Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Atlanta Department of Veterans Affairs Medical Center  Decatur Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/52.2.214"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jpen.1669"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12353"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665115000129"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199109053251005"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.45.1.459"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2018.11.021"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2018.11.021"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jpen.1312"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMct0806956"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-072610-145159"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx656"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrestox.6b00179"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0000000000000451"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/0148607116656164"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-018-1767-1"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040009"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2015.85"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glt152"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0000000000000578"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1519\/JSC.0b013e31829eef24"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glw046"},{"issue":"10","key":"e_1_2_10_24_1","first-page":"1352","article-title":"Metabolites associated with lean mass and adiposity in older Black men","volume":"72","author":"Murphy RA","year":"2017","journal-title":"J Gerontol A Biol Sci Med Sci"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101574"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jand.2020.07.020"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.114.088831"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2017.02.006"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/oby.22654"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-15"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp291"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c00338"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/es5002105"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-007-0082-2"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003123"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyz273"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-45328-2_1"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.575586"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2015.07.001"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812874106"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2012.01.024"},{"volume-title":"Biochemistry","year":"2014","author":"Ferrier DR","key":"e_1_2_10_43_1"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01435"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-012-2555-x"},{"key":"e_1_2_10_46_1","first-page":"331","volume-title":"Modern Nutrition in Health and Disease","author":"Kirkland JB","year":"2014"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.04.008"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098313"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b01214"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2017.09.015"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2015.12.010"}],"container-title":["Journal of Parenteral and Enteral Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jpen.2201","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jpen.2201","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aspenjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jpen.2201","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:52:22Z","timestamp":1760871142000},"score":1,"resource":{"primary":{"URL":"https:\/\/aspenjournals.onlinelibrary.wiley.com\/doi\/10.1002\/jpen.2201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,26]]},"references-count":50,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1002\/jpen.2201"],"URL":"https:\/\/doi.org\/10.1002\/jpen.2201","archive":["Portico"],"relation":{},"ISSN":["0148-6071","1941-2444"],"issn-type":[{"type":"print","value":"0148-6071"},{"type":"electronic","value":"1941-2444"}],"subject":[],"published":{"date-parts":[[2021,7,26]]},"assertion":[{"value":"2021-07-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}