{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:31:37Z","timestamp":1782282697602,"version":"3.54.5"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T00:00:00Z","timestamp":1641254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aspenjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Parenter Enteral Nutr"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Sepsis is a lethal syndrome with T\u2010cell dysregulation, imbalanced inflammatory reactions, and gastrointestinal dysfunction. Obesity coexistent with sepsis can cause more\u2010deleterious disease outcomes. Vitamin D is a nutrient with immunomodulatory ability and helps maintain intestinal homeostasis. This study investigated treatment with calcitriol on mesenteric lymph node (MLN) CD4<jats:sup>+<\/jats:sup> T\u2010cell polarization and intestinal injury in obese mice with sepsis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice received a high\u2010fat diet for 10 weeks; then, mice were separated into an obese control group without sepsis and sepsis groups that underwent cecal ligation and puncture (CLP). Septic mice were subdivided into a group that was injected with saline (SS group) or a group that was injected with calcitriol (SD group) via a tail vein 1 h after CLP. Obese mice with sepsis were euthanized at 12 or 24 h post CLP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sepsis resulted in increased percentages of type 2 T helper (Th2), Th17, and regulatory T (Treg) cells in MLNs. Also, inflammation\u2010associated genes were upregulated and tight junction genes downregulated in the intestines after CLP. Compared with the SS group, the SD group exhibited reduced Th2, Th17, and Treg percentages in MLNs. Also, intestinal inflammatory chemokine expressions were reduced, whereas <jats:italic>MUC2<\/jats:italic>, <jats:italic>ZO\u20101<\/jats:italic>, and <jats:italic>occludin<\/jats:italic> had increased after CLP. Lower inflammatory cytokine levels in peritoneal lavage fluid in the ileum were also noted in the SD group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Intravenous calcitriol treatment after sepsis can elicit more\u2010balanced CD4 T\u2010cell subsets in lymph nodes near the intestines and alleviate intestinal inflammation and injury in obese mice complicated with sepsis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jpen.2313","type":"journal-article","created":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T03:28:51Z","timestamp":1639020531000},"page":"1371-1383","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Intravenous calcitriol administration modulates mesenteric lymph node CD4<sup>+<\/sup> T\u2010cell polarization and attenuates intestinal inflammation in obese mice complicated with polymicrobial sepsis"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8245-4601","authenticated-orcid":false,"given":"Chiu\u2010Li","family":"Yeh","sequence":"first","affiliation":[{"name":"School of Nutrition and Health Sciences College of Nutrition Taipei Medical University  Taipei Taiwan"},{"name":"Nutrition Research Center Taipei Medical University Hospital  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Ming","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Surgery, National Taiwan University Hospital and College of Medicine National Taiwan University  Taipei Taiwan"},{"name":"Department of Surgery National Taiwan University Hospital Hsin\u2010Chu Branch  Hsin\u2010Chu City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po\u2010Jen","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Surgery, National Taiwan University Hospital and College 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