{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T12:24:50Z","timestamp":1778070290648,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T00:00:00Z","timestamp":1708646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aspenjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Parenter Enteral Nutr"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Central line\u2013associated bloodstream infections are a major concern for children with intestinal failure and in animal research using parenteral nutrition (PN). In neonatal piglets receiving PN, we compared sepsis, line occlusions, line replacements, mortality, and costs with and without the use of a 4%\u2010tetrasodium ethylenediaminetetraacetic acid (T\u2010EDTA) locking solution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed a retrospective review of piglets with a central venous jugular catheter enrolled in 14\u2010day exclusive PN (TPN) trials or in 7\u2010day short bowel syndrome (SBS) trials, before and after initiation of T\u2010EDTA. Lines were locked with a 1\u2010ml solution for 2\u2009h daily (T\u2010EDTATPN, <jats:italic>n<\/jats:italic>\u2009=\u200917; T\u2010EDTASBS, <jats:italic>n<\/jats:italic>\u2009=\u200948) and compared with our prior standard of care using 1.5\u2010ml heparin flushes twice daily (CONTPN, <jats:italic>n<\/jats:italic>\u2009=\u200934; CONSBS, <jats:italic>n<\/jats:italic>\u2009=\u200948). Line patency and signs of sepsis were checked twice daily. Jugular catheters were replaced for occlusions whenever possible. Humane end points were used for sepsis not responding to antibiotic treatment or unresolved catheter occlusions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with CON, sepsis was reduced using T\u2010EDTA, significantly for TPN (<jats:italic>P<\/jats:italic>\u2009=\u20090.006) and with a trend for SBS piglets (<jats:italic>P<\/jats:italic>\u2009=\u20090.059). Line occlusions necessitating line changes were reduced 15% in TPN studies (<jats:italic>P<\/jats:italic>\u2009=\u20090.16), and no line occlusions occurred for T\u2010EDTA SBS piglets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In our neonatal piglet research, use of T\u2010EDTA locking solution decreased sepsis and, although not statistically significant, reduced occlusions requiring line replacements. Given the expense of animal research, adding a locking solution must be cost\u2010effective, and we were able to show that T\u2010EDTA significantly reduced total research costs and improved animal welfare.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jpen.2609","type":"journal-article","created":{"date-parts":[[2024,2,24]],"date-time":"2024-02-24T02:01:09Z","timestamp":1708740069000},"page":"495-501","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Association between 4%\u2010tetrasodium EDTA and sepsis in neonatal piglets: A retrospective cohort study"],"prefix":"10.1002","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8700-0035","authenticated-orcid":false,"given":"Mirielle L.","family":"Pauline","sequence":"first","affiliation":[{"name":"Department of Pediatrics University of Alberta Edmonton Alberta 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