{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T19:46:18Z","timestamp":1790451978660,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82401137"],"award-info":[{"award-number":["82401137"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Horizontal platelet\u2010rich fibrin (H\u2010PRF) is a novel platelet concentrate known for promoting tissue regeneration, the purpose of this study was to assess the impact of H\u2010PRF on gingival defects healing using both in vitro and in vivo approaches.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Human gingival fibroblasts (hGFs) were cultured with lipopolysaccharide (LPS), H\u2010PRF, and H\u2010PRF + LPS. Then, cell viability, proliferation, apoptosis and migration were evaluated via fluorescence staining, CCK\u20108, flow cytometry, scratch and Transwell assays, respectively. Gingival defects were created in a rabbit model and treated with or without H\u2010PRF membranes. Tissue remodeling was evaluated at 14 and 21 days post\u2010surgery using hematoxylin and eosin (H&amp;E) staining, Masson's trichrome staining, and immunohistochemistry (IHC) for E\u2010cadherin, and neovascularization was evaluated by IHC for CD31.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>H\u2010PRF promoted hGFs proliferation and migration and mitigated the negative effects of LPS on hGF viability and apoptosis. In animal model, H\u2010PRF significantly accelerated gingival defect closure, reduced pseudomembrane formation, and promoted the formation of a thicker, stratified squamous epithelium with rete peg formation by day 21. Compared with untreated defects, H\u2010PRF\u2010treated defects exhibited significantly increased collagen deposition and elevated expression of E\u2010cadherin. Moreover, enhanced neovascularization was observed in the H\u2010PRF group, as evidenced by the increased expression of CD31\u2010positive cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>H\u2010PRF significantly improved gingival defects healing by enhancing hGF cellular behaviors and promoting epithelialization and neovascularization. These findings support the potential of H\u2010PRF as an effective adjunctive therapy during gingival tissue regeneration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Plain Language Summary<\/jats:title>\n                    <jats:p>Horizontal platelet\u2010rich fibrin (H\u2010PRF) is a platelet concentrate derived from patient's own blood that has gained momentum in regenerative medicine due to its ability to support tissue repair. In our study, we compared the healing process of gingival defects treated with H\u2010PRF to those that healed naturally and found that the defects treated with H\u2010PRF healed more quickly and more completely. The treated areas contained stronger and more organized tissue, with improved blood flow. H\u2010PRF also helps the cells in the gums move to the injury site more effectively, which is important for faster and more complete healing. Our findings suggest that H\u2010PRF could be a useful tool to enhance the outcomes of soft tissue augmentation around natural teeth or implants, which would lead to healthier and stronger gingiva for patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jper.11364","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T13:53:22Z","timestamp":1751032402000},"page":"486-497","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Efficacy of horizontal platelet\u2010rich fibrin on gingival tissue regeneration: Cellular and histological analysis"],"prefix":"10.1002","volume":"97","author":[{"given":"Jihua","family":"Chai","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6729-8549","authenticated-orcid":false,"given":"Yulu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Stomatology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Shi","sequence":"additional","affiliation":[{"name":"The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture  Enshi Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaibo","family":"Yang","sequence":"additional","affiliation":[{"name":"The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture  Enshi Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sensen","family":"Li","sequence":"additional","affiliation":[{"name":"The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture  Enshi Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J.","family":"Miron","sequence":"additional","affiliation":[{"name":"Department of Periodontology University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12957"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12952"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12415"},{"key":"e_1_2_9_5_1","first-page":"17","article-title":"Soft tissue substitutes in periodontal and peri\u2010implant soft tissue augmentation: a systematic review","author":"Rotundo R","year":"2024","journal-title":"Materials (Basel)"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/clr.14150"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/clr.13114"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.19-0716"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.19-0350"},{"key":"e_1_2_9_10_1","first-page":"144","article-title":"Autologous platelet concentrates in regenerative dentistry\u2014A narrative literature review. 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