{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:01:36Z","timestamp":1791810096312,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,4,23]],"date-time":"2018-04-23T00:00:00Z","timestamp":1524441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aap.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The purpose of this study was to characterize and compare subgingival and salivary microbiotas before and after periodontal treatment to learn if any changes of the subgingival microbiota were reflected in saliva. We tested the hypothesis that salivary levels of specific periopathogens correlate with corresponding subgingival levels before and after periodontal treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010five patients with generalized chronic periodontitis completed the study. Stimulated saliva samples and subgingival plaque samples were collected at baseline and 2, 6, and 12\u00a0weeks after nonsurgical periodontal therapy. Subgingival and salivary microbiotas were processed by means of the Human Oral Microbe Next Generation Sequencing (HOMI<jats:italic>NGS<\/jats:italic>) technique and characterized based on relative abundance. Spearman signed rank test was used to test correlation of periopathogens in subgingival and saliva samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Periodontal treatment resulted in significantly higher relative abundance of <jats:italic>Streptococcus, Rothia<\/jats:italic> and <jats:italic>Actinomyces<\/jats:italic> in combination with a significant decrease in <jats:italic>Porphyromonas<\/jats:italic> and <jats:italic>Treponema<\/jats:italic> in subgingival plaque samples. Relative abundance of the overall predominant genera in saliva was not influenced by periodontal treatment. However, there was a positive correlation between samples of subgingival plaque and saliva before and after periodontal treatment (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0001) with respect to relative abundance of specific periopathogens, such as <jats:italic>Porphyromonas gingivalis<\/jats:italic> (r\u00a0=\u00a00.68), <jats:italic>Prevotella intermedia<\/jats:italic> (r\u00a0=\u00a00.72), <jats:italic>Filifactor alocis<\/jats:italic> (r\u00a0=\u00a00.58), <jats:italic>Treponema denticola<\/jats:italic> (r\u00a0=\u00a00.51), <jats:italic>Tannerella forsythia<\/jats:italic> (r\u00a0=\u00a00.45) and <jats:italic>Parvimonas micra<\/jats:italic> (r\u00a0=\u00a00.45).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Subgingival and salivary abundance of periodontal pathogens correlated before and after treatment. Thus, data from this study suggest that periopathogens identified in saliva may be spill\u2010over from the subgingival microbiota.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jper.17-0377","type":"journal-article","created":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T13:36:32Z","timestamp":1519220192000},"page":"531-539","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":98,"title":["Influence of periodontal treatment on subgingival and salivary microbiotas"],"prefix":"10.1002","volume":"89","author":[{"given":"Daniel","family":"Belstr\u00f8m","sequence":"first","affiliation":[{"name":"Section for Periodontology Microbiology and Community Dentistry Department of Odontology Faculty of Health Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Anastasia","family":"Grande","sequence":"additional","affiliation":[{"name":"Section for Periodontology Microbiology and Community Dentistry Department of Odontology Faculty of Health Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Lynn","family":"Sembler\u2010M\u00f8ller","sequence":"additional","affiliation":[{"name":"Section for Oral Medicine Department of Odontology Faculty of Health Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolai","family":"Kirkby","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean L.","family":"Cotton","sequence":"additional","affiliation":[{"name":"The Forsyth Institute  Cambridge MA United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce J.","family":"Paster","sequence":"additional","affiliation":[{"name":"The Forsyth Institute  Cambridge MA United States"},{"name":"Department of Oral Medicine Infection &amp; Immunity Harvard School of Dental Medicine  Boston MA United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Palle","family":"Holmstrup","sequence":"additional","affiliation":[{"name":"Section for Periodontology Microbiology and Community Dentistry Department of Odontology Faculty of Health Sciences University of Copenhagen  Copenhagen 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